Porous mirror for optical detection of an analyte in a fluid

Patent metadata

Field	Value
Publication number	US-11079319-B2
Application number	US-202016854136-A
Country	US
Kind code	B2
Filing date	Apr 21, 2020
Priority date	Nov 18, 2015
Publication date	Aug 3, 2021
Grant date	Aug 3, 2021

Abstract

A porous mirror (1) for detection of an analyte (96) in a fluid (99) by optical probing, comprising a translucent slab (2) with a front side (3), and a backside (4) facing away from the front side (3), wherein the front side (3) is adapted for being contacted with a fluid (99), and a reflective layer (5) at the front side (3) of the translucent slab (2), the reflective layer (5) being adapted to reflect light reaching the reflective layer from the backside (4) of the translucent slab (2), wherein the translucent slab (2) comprises pores (6), wherein the pores (6) are dead end pores (6) extending from respective openings (7) at the front side (3) into the translucent slab (2), through the reflective layer (5), wherein a cross-sectional dimension of the openings (7) of the pores (6) is dimensioned so as to prevent larger particles or debris, if any included the fluid, from entering the pores (6), while allowing the analyte (96) in the fluid (99) to enter the pores (6) via diffusion.

First claim

The invention claimed is:

1. A device for detecting an analyte in a fluid comprising a porous mirror comprising:
a translucent article comprising (a) at least one membrane comprising pores and (b) a transparent element with a front side, and a backside facing away from the front side, wherein the front side is in contact with a fluid chamber;
a reflective layer at the front side of the translucent article, which reflects light reaching the reflective layer from the backside of the translucent article;
wherein the pores are dead end pores extending from respective openings at the front side into the at least one membrane, through the reflective layer;
wherein the pores are only in fluid communication with the fluid at the front side of the at least one membrane;
a light source, to illuminate at least the pores in the translucent article; and
a light detector, wherein the detector is arranged to receive light emerging from the pores in response to an illumination by the light source, and wherein the detector generates a signal representative of the detected light.

2. The device of claim 1 , wherein the fluid chamber comprises an inlet and outlet port for feeding and discharging the fluid.

3. The device of claim 1 , further comprising one or more further detectors.

4. The device of claim 1 , wherein a cross-sectional dimension of the openings of the pores is about 1 μm or less and/or wherein a length of the pores in an axial direction along the pores is less than 100 μm.

5. The device of claim 1 , wherein a porosity of a given volume of the translucent article is between 50% and 5% by volume.

6. The device of claim 1 , wherein an equivalent pore volume depth (DELTA) is less than 20 μm, wherein the equivalent pore volume depth (DELTA) is defined as the total volume of the pores (V) divided by the front side area (A) over which the openings of the pores are distributed.

7. The device of claim 1 , wherein an inner wall surface of the pores is coated with a hydrophilic coating.

8. The device of claim 1 , wherein the translucent article is provided with further reflective elements arranged inside the pores, in a mouth portion thereof, adjacent to the opening at the front side of the translucent article.

9. The device of claim 8 , wherein the further reflective elements are provided as a reflective coating covering only a fraction of the circumference of the mouth portion of the pores in the vicinity of the opening, wherein the fraction is about 70% or less.

10. The device of claim 1 , wherein a transparent backing side of the translucent article is provided with a 60° angled surface to minimize the effect of the shift in refractive index between outside air and the transparent backing slide.

11. The device of claim 1 , wherein the pores are track-etched in the translucent article and reflective layer.

12. The device of claim 1 , wherein the pores are rinsed by diffusion.

13. The device of claim 1 , wherein at least one of the at least one membranes comprising pores is made of a transparent polymer.

14. The device of claim 1 , wherein a cross-sectional dimension of the openings of the pores is about 500 nm or less and/or wherein a length of the pores in an axial direction along the pores is less than 50 μm.

15. The device of claim 1 , wherein a porosity of a given volume of the translucent article is between 30% and 10% by volume.

16. The device of claim 1 , wherein an equivalent pore volume depth (DELTA) is less than 10 μm, wherein the equivalent pore volume depth (DELTA) is defined as the total volume of the pores (V) divided by the front side area (A) over which the openings of the pores are distributed.

17. The device of claim 8 , wherein the further reflective elements are provided as a reflective coating covering only a fraction of the circumference of the mouth portion of the pores in the vicinity of the opening, wherein the fraction is about 50% or less.

18. The device of claim 1 , wherein the pores are track-etched pores.

19. The device of claim 18 , wherein at least one of the at least one membranes comprising pores translucent slab is made of a transparent polymer.

20. The device of claim 1 , wherein the fluid is a whole blood sample, the plasma fraction of whole blood, spinal cord fluid, urine, pleura, ascites, wastewater, a pre-prepared fluid for any kind of injection, a fluid with a constituent detectable by spectroscopy, or a gas.

21. The device of claim 1 , wherein the reflective layer is made of metal.

22. A method for optically detecting an analyte in a fluid comprising
providing the device according to claim 1 ,
contacting the porous mirror with a reference liquid so as to fill the pores with the reference liquid,
contacting the front side of the porous mirror with the fluid,
waiting for a diffusion time to allow for diffusion of the analyte in the fluid into the pores to stabilize,
optically probing the fluid inside the pores from the side of the reflective layer, and
based on the result of the optical probing, establishing an analyte level of the fluid.

23. The method according to claim 22 , wherein the analyte is bilirubin, carbon dioxide, Patent Blue V, or methylene blue and the fluid is whole blood.

24. The method according to claim 22 , wherein the fluid is a whole blood sample, the plasma fraction of whole blood, spinal cord fluid, urine, pleura, ascites, wastewater, a pre-prepared fluid for any kind of injection, a fluid with a constituent detectable by spectroscopy, or a gas.

Assignees

• Radiometer Medical Aps

Inventors

• Kjaer Thomas

Classifications

• G01N2015/012

  Red blood cells · CPC title

• G01N15/01

  specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05) · CPC title

• G01N21/03

  Cuvette constructions · CPC title

• G01N21/31 Primary

  Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry {(G01N21/72 takes precedence)} · CPC title

• G02B5/0808 Primary

  having a single reflecting layer (G02B5/0883, G02B5/0891 take precedence) · CPC title