Antimicrobial peptide from skate skin and uses thereof

Patent metadata

Field	Value
Publication number	US-11077170-B2
Application number	US-201816648043-A
Country	US
Kind code	B2
Filing date	Sep 7, 2018
Priority date	Sep 22, 2017
Publication date	Aug 3, 2021
Grant date	Aug 3, 2021

Abstract

A method for treating a microorganism or inflammation caused by the microorganism includes administering or applying a composition comprising an antimicrobial peptide with the amino acid sequence of SEQ ID NO: 1 to a subject in need thereof. Because the antimicrobial peptide with the amino acid sequence of SEQ ID NO: 1 exhibits an excellent antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria and also low cytotoxicity for human erythrocytes, it can be advantageously used for various applications.

First claim

The invention claimed is:

1. A method for treating a microorganism infection or inflammation caused by the microorganism, the method comprising administering or applying a composition comprising an antimicrobial peptide with the amino acid sequence of SEQ ID NO: 1 to a subject in need thereof.

2. The method of claim 1 , wherein the microorganism comprises at least one of Gram-negative bacteria and Gram-positive bacteria.

3. The method of claim 1 , wherein the microorganism comprises Gram-negative bacteria selected from the group consisting of Escherichia coli, Pseudomonas aeruginosa , and Acinetobacter baumannii.

4. The method of claim 1 , wherein the microorganism comprises Escherichia coli.

5. The method of claim 1 , wherein the microorganism comprises Pseudomonas aeruginosa.

6. The method of claim 1 , wherein the microorganism comprises Acinetobacter baumannii.

7. The method of claim 1 , wherein the microorganism comprises Staphylococcus aureus.

8. The method of claim 1 , wherein the composition is an antibiotic composition comprising the antimicrobial peptide and additional antibiotics other than the antimicrobial peptide.

9. The method of claim 8 , wherein the antibiotics comprises at least one selected from the group consisting of aminoglycoside-based antibiotics, penicillin-based antibiotics, sulfonamide-based antibiotics, beta-lactam based antibiotics, chloramphenicol-based antibiotics, erythromycin-based antibiotics, florfenicol-based antibiotics, fosfmycin-based antibiotics, kanamycin-based antibiotics, lincomycin-based antibiotics, meticillin-based antibiotics, quinolone-based antibiotics, streptomycin-based antibiotics, tetracycline-based antibiotics, trimethoprim-based antibiotics, and vancomycin-based antibiotics.

10. The method of claim 1 , wherein the composition is a quasi-drug composition comprising the antimicrobial peptide and a quasi-drug component.

11. The method of claim 10 , wherein quasi-drug component comprises at least one selected from the group consisting of a sterilizing cleanser, a shower foam, a mouth wash, a water tissue, a liquid soap, a hand wash, a humidifier filler, a mask, an ointment, a patch, and a filter filler.

12. The method of claim 1 , wherein the composition is a cosmetic composition comprising the antimicrobial peptide and a cosmetic component.

13. The method of claim 12 , wherein the cosmetic component comprises at least one selected from the group consisting of an anti-oxidant, a stabilizing agent, a solubilizing agent, a vitamin, a pigment, a fragrance, and a carrier.

14. The method of claim 12 , wherein the antimicrobial peptide is in an amount of 0.1 to 50% by weight based on the total weight of the antibiotic cosmetic composition.

15. The method of claim 12 , wherein the cosmetic composition is in a formulation selected from the group consisting of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleanser, an oil, a powder foundation, an emulsion foundation, a wax foundation, and a spray.

16. The method of claim 1 , wherein the composition is a food comprising the antimicrobial peptide and a food additive component.

17. The method of claim 1 , wherein the composition is an animal feed comprising the antimicrobial peptide and an animal feed component.

18. The method of claim 1 , wherein the composition is an antibiotic biopesticides comprising the antimicrobial peptide and a biopesticide component.

19. The method of claim 1 , wherein the composition is a preservative composition comprising the antimicrobial peptide and a preservative agent.

Assignees

• Univ Chosun Iacf

Inventors

• Park Yoonkyung
• Lee Jong-Kook

Classifications

• A23B2/7295

  Antibiotics · CPC title

• A23B2/762

  Organic compounds containing nitrogen · CPC title

• A61Q19/00

  Preparations for care of the skin · CPC title

• A61K38/1706 Primary

  from fish · CPC title

• A23K50/10

  for ruminants · CPC title