L-amino acid-containing feedstuff additive

Patent metadata

Field	Value
Publication number	US-11076616-B2
Application number	US-201415031260-A
Country	US
Kind code	B2
Filing date	Oct 6, 2014
Priority date	Oct 24, 2013
Publication date	Aug 3, 2021
Grant date	Aug 3, 2021

Abstract

By substituting biomass with a surface-active substance in a fermentation broth containing amino acid before spray-drying, granulated animal feed additives with improved product specifications were obtained.

First claim

The invention claimed is:

1. A method for preparing a feed additive, comprising the following steps:
a) obtaining a low biomass content fermentation broth by:
i) fermenting an L-amino acid-producing microorganism in an aqueous culture medium to produce a fermentation broth;
ii) after the fermenting of step i) is complete:
aa) removing biomass from the fermentation broth to reduce the biomass content to, at most, 4% by weight;
bb) adding surface-active substance to the fermentation broth to a final concentration of 0.025 to 20% by weight; and

b) drying the low biomass content fermentation broth obtained in step a) in order to convert it into said feed additive;

wherein the concentrations of biomass and surface-active substance in the low biomass content fermentation broth are such that the feed additive of step b) is a particulate composition with a particle density of at least 1.20 g/cm 3 .

2. The method of claim 1 , wherein the concentrations of biomass and surface-active substance in the low biomass content fermentation broth are such that the feed additive of step b) is a particulate composition with a particle density of 1.20-1.30 g/cm 3 .

3. The method of claim 1 , further comprising completely or partially coating the particulate composition with an edible oil.

4. The method of claim 1 , wherein the L-amino acid is L-lysine, L-methionine, L-threonine, L-valine or L-tryptophan.

5. The method of claim 1 , wherein the amino acid is L-lysine and wherein, in a step after completion of fermentation and before the start of drying, ammonium sulphate and/or sulphuric acid is added to the fermentation broth in order to establish a sulphate/L-amino acid ratio of 0.85 to 1.2.

6. The method of claim 1 , wherein at least 30% by weight of the biomass is removed from the fermentation broth before drying.

7. The method of claim 1 , wherein the surface-active substance is selected from the group consisting of: corn steep liquor; lipids; antifoaming agents; surfactants; and mixtures thereof.

8. The method of claim 1 , wherein the surface-active substance is an antifoaming agent selected from the group consisting of: polysiloxanes; mono- and polyglycols; phospholipids; and fatty acid glycerides.

9. The method of claim 1 , wherein the fermentation broth used in the drying comprises:
a) an L-amino acid content, as amino acid base, of 12 to 48% by weight;
b) a solids content, including biomass, of 20 to 60% by weight;
c) a surface-active substance content of 0.025 to 20% by weight;
d) a sulphate to L-amino acid ratio of 0.8 to 1.2; and
e) a pH of 3.5 to 7.0.

10. The method of claim 1 , wherein drying is conducted by spray-drying.

11. The method of claim 1 , wherein the fermentation broth used in the drying step comprises:
a) a biomass content of at most 1% by weight;
b) an L-lysine content, as amino acid base, of 12 to 48% by weight;
c) a solids content, including biomass, of 30 to 50% by weight;
d) a surface-active substance content of 0.3 to 10% by weight;
e) a sulphate to L-lysine ratio of 0.8 to 1.2; and
f) a pH of 4.0 to 5.0.

12. The method of claim 1 , wherein:
a) at least 50% by weight of the biomass is removed from the fermentation broth before drying;
b) the surface-active substance is present in the fermentation broth before drying in an amount of 0.3 to 10% by weight;
c) drying is conducted by spray granulation using a fluidized bed reactor;
d) the L-amino acid is L-lysine.

13. The method of claim 12 , further comprising completely or partially coating the particulate composition with an edible oil.

14. The method of claim 13 , wherein, in a method step after completion of the fermentation and before the start of the drying, ammonium sulphate and/or sulphuric acid is added to the fermentation broth in order to establish a sulphate/L-amino acid ratio of 0.85 to 1.2.

15. The method of claim 14 , wherein at least 70% by weight of the biomass is removed from the fermentation broth before drying and wherein said feed additive is a particulate composition with a particle density of 1.20-1.30 g/cm 3 .

16. The method of claim 15 , wherein at least 90% by weight of the biomass is removed from the fermentation broth before drying.

17. The method of claim 1 , wherein the fermentation broth is converted directly into a granulate in one process step, and wherein the granulate comprises: 40-60% L-lysine; a mean particle diameter of 60-1500 μm; a biomass content of 0.1-2% by weight; a surface-active substance content of 0.5 to 15% by weight; and a water content of at most 3.5% by weight.

18. A method for preparing a feed additive, comprising the following steps:
a) fermenting an L-amino acid acid-producing microorganism in an aqueous culture medium to produce a fermentation broth;
b) after the fermenting of step a) is complete, converting the fermentation broth into a low biomass content fermentation broth by:
i) removing biomass from the fermentation broth to reduce the biomass content to, at most, 4% by weight, wherein at least 50% by weight of the biomass is removed from the fermentation broth before drying;
ii) adding a surface-active substance to the fermentation broth, wherein said surface-active substance is added to a final concentration of 0.025 to 20% by weight; and

c) drying the low biomass content fermentation broth obtained in step b) to convert it into a said feed additive;

wherein the concentrations of biomass and surface-active substance in the low biomass content fermentation broth are such that the feed additive of step c) is a particulate composition with a particle density of 1.20-1.30 g/cm 3 .

19. The method of claim 18 , wherein, in a step after completion of fermentation and before the start of drying, ammonium sulphate and/or sulphuric acid is added to the fermentation broth in order to establish a sulphate/L-amino acid ratio of 0.85 to 1.2.

20. The method of claim 18 wherein, said L-amino acid is L-lysine and said surface-active substance is selected from the group consisting of: corn steep liquor; lipids; antifoaming agents; surfactants; and mixtures thereof.

Assignees

• Evonik Operations Gmbh

Inventors

• Oelmann Ansgar
• Alt Hans Christian
• Becker Franz Ulrich
• Blümke Wilfried
• Claes Wilfried

Classifications

• C12P13/12

  Methionine; Cysteine; Cystine · CPC title

• A23K40/10 Primary

  by agglomeration; by granulation, e.g. making powders · CPC title

• A23K40/30

  by encapsulating; by coating · CPC title

• C12P13/08

  Lysine; Diaminopimelic acid; Threonine; Valine · CPC title

• A23K20/158

  Fatty acids; Fats; Products containing oils or fats · CPC title