Endophytic microbial symbionts in plant prenatal care

Patent metadata

Field	Value
Publication number	US-11076573-B2
Application number	US-201816141859-A
Country	US
Kind code	B2
Filing date	Sep 25, 2018
Priority date	Feb 5, 2013
Publication date	Aug 3, 2021
Grant date	Aug 3, 2021

Abstract

The present disclosure provides compositions comprising novel endophytes capable of promoting germination endophytes that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom.

First claim

The invention claimed is:

1. A method of increasing resistance to nitrogen stress in at least one agricultural crop, said method comprising mechanically or manually inoculating a seed of the agricultural crop with a composition comprising at least one endophyte and a carrier, wherein the at least one endophyte is of genus Streptomyces and comprises a 16S rDNA sequence with 100% sequence identity to SEQ ID NO:6 or is deposited as IDAC 081111-06 and is capable of capable of causing populations of seeds to have increased yield under nitrogen stress conditions compared to control seeds.

2. The method of claim 1 , wherein the endophyte is deposited as IDAC 081111-06.

3. The method of claim 1 , wherein the endophyte comprises a 16S rDNA sequence with 100% sequence identity to SEQ ID NO:6.

4. The method of claim 1 , wherein the agricultural crop is selected from the group consisting of cereal, pulse, and canola.

5. The method of claim 1 , wherein the crop is a cereal.

6. The method of claim 1 , wherein the crop is wheat.

7. The method of claim 1 , wherein the seed is a durum wheat cultivar AC Avonlea seed.

8. The method of claim 1 , wherein the nitrogen stress conditions result from lack of application of nitrogen to a field.

9. The method of claim 1 , wherein the nitrogen stress conditions result from field nitrogen not present at agriculturally relevant rates.

10. The method of claim 1 , wherein manually or mechanically inoculating the seed comprises coating the seed with the endophyte.

11. The method of claim 1 , wherein the inoculated seed is coated with at least 100 CFU of the endophyte per seed.

12. The method of claim 1 , wherein the carrier is a liquid carrier.

13. The method of claim 1 , wherein the endophyte colonizes at least 1% of the cortical cells of an agricultural plant grown from the inoculated seed.

14. The method of claim 1 , wherein the endophyte is a spore.

15. The method of claim 1 , further comprising cultivating the inoculated seed into a first generation plant.

16. The method of claim 1 , wherein the endophyte is deposited as IDAC 081111-06, the crop is wheat, and manually or mechanically inoculating the seed comprises coating the seed with the endophyte.

Assignees

• Univ Saskatchewan

Inventors

• Vujanovic Vladimir
• Germida James J

Classifications

• A01N63/30

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• C12R2001/80

  Penicillium · CPC title

• C12R2001/465

  Streptomyces · CPC title

• A01N63/28

  Streptomyces · CPC title

• A01N25/00

  Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests · CPC title