Multisignal reagents for labeling analytes

Patent metadata

Field	Value
Publication number	US-11073512-B2
Application number	US-201816202213-A
Country	US
Kind code	B2
Filing date	Nov 28, 2018
Priority date	Apr 3, 2003
Publication date	Jul 27, 2021
Grant date	Jul 27, 2021

Abstract

Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.

First claim

What is claimed is:

1. A DNA oligonucleotide having a 5′ end and a 3′ end, comprising:
a first member of a first binding pair attached at the 5′ end of the DNA oligonucleotide, wherein the first binding pair is an antigen-antibody binding pair; and
a first member of a second binding pair attached at the 3′ end of the DNA oligonucleotide, wherein the second binding pair is an antigen-antibody binding pair.

2. The DNA oligonucleotide of claim 1 , wherein the first member of the first binding pair is a protein.

3. The DNA oligonucleotide of claim 2 , wherein the first member of the first binding pair is an antibody.

4. The DNA oligonucleotide of claim 1 , wherein the first member of the second binding pair is a protein.

5. The DNA oligonucleotide of claim 4 , wherein the first member of the second binding pair is an antibody.

6. The DNA oligonucleotide of claim 3 , wherein the first member of the second binding pair is an antibody.

Assignees

• Enzo Life Sciences Inc

Inventors

• Coleman Jack
• Szczepanik Maciej
• Jin Richard

Classifications

• C12Q1/6818

  involving interaction of two or more labels, e.g. resonant energy transfer · CPC title

• C07H21/00

  Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids · CPC title

• C07H21/04

  with deoxyribosyl as saccharide radical · CPC title

• C09B11/24

  Phthaleins containing amino groups {; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes} · CPC title

• G01N33/581

  with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates) · CPC title