Compositions and methods comprising the use of exiguobacterium acetylicum and bacillus coagluans alpha-glucanotransferase enzymes

Patent metadata

Field	Value
Publication number	US-11072783-B2
Application number	US-201615760055-A
Country	US
Kind code	B2
Filing date	Oct 6, 2016
Priority date	Oct 6, 2015
Publication date	Jul 27, 2021
Grant date	Jul 27, 2021

Abstract

An isolated and/or purified α-glucanotransferase from Exiguobacterium Acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the α-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the α-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.

First claim

What is claimed is:

1. A composition comprising at least: (i) an isolated GH70 subfamily 4 α-glucanotransferase comprising an amino acid sequence that is at least 95% identical to amino acids 31-731 of SEQ ID NO:2, and (ii) a glucooligosaccharide with α-(1→4) and α-(1→6) glycosidic linkages.

2. The composition of claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence that is at least 98% identical to amino acids 31-731 of SEQ ID NO:2.

3. The composition of claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence that is at least 99% identical to amino acids 31-731 of SEQ ID NO:2.

4. The composition of claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence that is at least 99.5% identical to amino acids 31-731 of SEQ ID NO:2.

5. The composition of claim 1 , wherein the α-glucanotransferase comprises amino acids 31-731 of SEQ ID NO:2.

6. The composition of claim 1 , wherein the composition is in a lyophilized powder form, an encapsulated form, a coated form, a granulated form, or a liquid formulation.

7. The composition of claim 1 , further comprising a diluent.

8. A method of producing saccharide products, said method comprising contacting the α-glucanotransferase according to claim 1 with a substrate comprising a maltooligosaccharide or maltodextrin under conditions of pH 3-10 and 30-70° C. to produce at least one glucooligosaccharide with α-(1→4) and α-(1→6) glycosidic linkages.

9. The method of claim 8 , wherein the substrate comprises malto-tetraose, malto-pentaose, malto-hexaose, or maltoheptaose.

10. The method of claim 8 , wherein the substrate is comprised in a beverage or food, wherein said glucooligosaccharide with α-(1→4) and α-(1→6) glycosidic linkages is produced in the beverage or food in situ.

11. The method of claim 10 , wherein the substrate is comprised in said beverage.

12. The method of claim 10 , wherein the substrate is comprised in said food.

Assignees

• Dupont Nutrition Biosci Aps

Inventors

• Kralj Slavko

Classifications

• C12N9/1074

  Cyclomaltodextrin glucanotransferase (2.4.1.19) · CPC title

• C12N9/1051 Primary

  Hexosyltransferases (2.4.1) · CPC title

• C12Y204/01025

  4-Alpha-glucanotransferase (2.4.1.25) · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• A23L33/10

  using additives (addition of substantially indigestible substances A23L33/21) · CPC title