Self-contained anaerobic culture device with microcompartments

Patent metadata

Field	Value
Publication number	US-11072771-B2
Application number	US-201615763744-A
Country	US
Kind code	B2
Filing date	Sep 27, 2016
Priority date	Sep 28, 2015
Publication date	Jul 27, 2021
Grant date	Jul 27, 2021

Abstract

In one aspect, the present disclosure provides a device. The device includes a body comprising a waterproof base, a waterproof coversheet attached to the base, and a channel disposed between the base and the coversheet. The channel has a perimeter and an opening that provides liquid access to the channel. A portion of the perimeter is defined by a waterproof seal. A dry first oxygen-scavenging reagent and an indicator reagent for detecting sulfate reduction by a sulfate-reducing bacterium are disposed in the device between the base and the coversheet. The waterproof base comprises a plurality of open microcompartment structures facing the coversheet.

First claim

The invention claimed is:

1. A device, comprising:
a body comprising a waterproof base, a waterproof coversheet attached to the waterproof base, and a channel disposed therebetween, the channel having a perimeter and a sealable opening that provides liquid access to the channel;
wherein a portion of the perimeter is defined by a waterproof seal; and

a dry first oxygen-scavenging reagent and an indicator reagent for detecting sulfate reduction by a sulfate-reducing bacterium are disposed in the device between the base and the coversheet;
wherein the waterproof base comprises a plurality of open microcompartment structures facing the coversheet.

2. The device of claim 1 , wherein the indicator reagent is disposed in the device adhered to the waterproof base or the waterproof coversheet.

3. The device of claim 1 , wherein the indicator reagent is the first a second oxygen-scavenging reagent.

4. The device of claim 1 , wherein the first oxygen-scavenging reagent is disposed in the device adhered to the waterproof base or the waterproof coversheet.

5. The device of claim 1 , further comprising a dry culture medium component disposed in the device between the base and the coversheet, the culture medium component selected to facilitate growth of a sulfate-reducing bacterium.

6. The device of claim 5 , wherein the culture medium component comprises an organic carbon source.

7. The device of claim 6 , wherein the organic carbon source is nonfermentable.

8. The device of claim 1 , further comprising a dry second oxygen-scavenging reagent disposed in the device between the base and the coversheet.

9. The device of claim 8 , wherein the second oxygen-scavenging reagent is disposed in the device adhered to the waterproof base or the waterproof coversheet.

10. The device of claim 1 , further comprising a reducing agent.

11. The device of claim 1 ;
wherein the waterproof base comprises a first adhesive layer disposed thereon facing the coversheet;
wherein a first dry component is adhered to the first adhesive layer;
wherein the first dry component is selected from the group consisting of the first oxygen-scavenging reagent, a second oxygen-scavenging reagent, a reducing agent, the indicator reagent, a culture medium component, and a combination of any two or more of the foregoing first dry components.

12. The device of claim 11 ;
wherein the waterproof coversheet comprises a second adhesive layer disposed thereon facing the base;
wherein a second dry component is adhered to the second adhesive layer;
wherein the second dry component is selected from the group consisting of the first oxygen-scavenging reagent, the second oxygen-scavenging reagent, the reducing agent, the indicator reagent, the culture medium component, and a combination of any two or more of the foregoing second dry components.

13. The device of claim 1 , wherein the first oxygen-scavenging reagent is water-soluble.

14. The device of claim 1 , wherein the first oxygen-scavenging reagent is selected from the group consisting of ferrous ammonium sulfate, ferrous chloride, ferrous sulfate, a ferrous iron salt, a metal sulfite, and a metal bisulfite.

15. The device of claim 8 , wherein the second oxygen-scavenging reagent is selected from the group consisting of ascorbic acid and salts thereof.

16. A method, comprising:
depositing a sample into the channel of the device of claim 1 ;
isolating separate portions of the sample into a plurality of the microcompartments, wherein isolating the separate portions comprises bonding the waterproof coversheet to the waterproof base such that a waterproof seal is formed and the separate portions are enclosed in separate microcompartments;
incubating the device for a period of time under conditions that facilitate growth of an anaerobic microorganism; and
detecting an indication of growth of the anaerobic microorganism in at least one of the separate microcompartments.

17. The method of claim 16 , further comprising depositing an aqueous liquid into the channel.

18. The method of claim 17 , wherein depositing the aqueous liquid into the channel comprises depositing a predetermined volume of the aqueous liquid.

19. The method of claim 18 , wherein depositing a predetermined volume comprises depositing about 0.1 mL to about 100 mL.

20. The method of claim 16 , wherein the method further comprises sealing the opening.

Assignees

• 3M Innovative Properties Co

Inventors

• Brutinel Evan D
• Bjork Jason W
• Stanenas Adam J

Classifications

• C12M41/34

  of gas · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• C12M23/12 Primary

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title

• C12Q1/04

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12M23/38

  Caps; Covers; Plugs; Pouring means · CPC title