Compositions and methods of making polymerizing nucleic acids

Patent metadata

Field	Value
Publication number	US-11072681-B2
Application number	US-201515329526-A
Country	US
Kind code	B2
Filing date	Jul 28, 2015
Priority date	Jul 28, 2014
Publication date	Jul 27, 2021
Grant date	Jul 27, 2021

Abstract

Provided herein are compositions and methods of making high density nucleic acid polymers.

First claim

What is claimed is:

1. A graft polymer comprising a linear backbone covalently bound to a plurality of oligonucleotide branches; wherein the graft polymer has the formula:
R 1 —[M(O)] n —R 2

wherein,
n is an integer from 2 to 1000;
M is a polymerized ROMP polymerizable monomer;
O is an oligonucleotide covalently attached directly to M or to M through a covalent linker; and
R 1 and R 2 are terminal polymer moieties,
wherein 100% of the polymerized ROMP polymerizable monomers are each individually attached directly to the oligonucleotide or to the oligonucleotide through a covalent linker, and
wherein the polymerizable monomer is N-substituted-5-norbornene-2,3-dicarboximide, wherein the substitution comprises the oligonucleotide.

2. The graft polymer of claim 1 , wherein said oligonucleotide comprises at least 3 nucleobases and at least 2 different nucleobases.

3. The graft polymer of claim 1 comprising at least 3 oligonucleotide branches.

4. The graft polymer of claim 1 , wherein R 1 and R 2 each independently comprise a substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl or substituted or unsubstituted heteroaryl.

5. The graft polymer of claim 1 , wherein M(O) is wherein,

L 1 is independently a bond, —O—, —NH—, —COO—, —S—, —SO 2 —, —SO 3 —, —SO 4 —, —SO 2 NH—, —NHC(O)—, —C(O)NH—, —NHC(O)O—, substituted or unsubstituted alkylene, substituted or unsubstituted heteroalkylene, substituted or unsubstituted cycloalkylene, substituted or unsubstituted heterocycloalkylene, substituted or unsubstituted arylene, or substituted or unsubstituted heteroarylene; and
R 4 is the oligonucleotide.

6. The graft polymer of claim 5 , wherein R 1 and R 2 each independently comprise a substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl or substituted or unsubstituted heteroaryl.

7. The graft polymer of claim 6 , wherein the oligonucleotide comprises at least 3 nucleobases and at least 2 different nucleobases.

Assignees

• Univ California

Inventors

• Rush Anthony M
• Gianneschi Nathan
• James Carrie

Classifications

• C07H21/00 Primary

  Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids · CPC title

• C08G2261/143

  Side-chains containing nitrogen · CPC title

• C08G2261/418

  Ring opening metathesis polymerisation [ROMP] · CPC title

• C08G61/02 Primary

  Macromolecular compounds containing only carbon atoms in the main chain of the macromolecule, e.g. polyxylylenes · CPC title

• C08G2261/3324

  derived from norbornene · CPC title