Method for preparing a steviol glycoside composition

Patent metadata

Field	Value
Publication number	US-11066688-B2
Application number	US-201615742561-A
Country	US
Kind code	B2
Filing date	Jul 11, 2016
Priority date	Jul 10, 2015
Publication date	Jul 20, 2021
Grant date	Jul 20, 2021

Abstract

The present invention relates to a composition comprising one or more steviol glycosides which composition comprises nitrogen in an amount of no more than about 1000 ppm. The invention also relates to a method for preparing a steviol glycoside composition, which method comprises: providing a steviol glycoside composition; combining the steviol glycoside composition with water to form a steviol glycoside solution; and crystallizing a steviol glycoside composition from the solution. The invention also relates to a method for reducing the nitrogen content of a steviol glycoside composition, which method comprises: providing a steviol glycoside composition which comprises nitrogen; combining the steviol glycoside composition with water to form a steviol glycoside solution; and crystallizing a steviol glycoside composition from the solution, thereby to reduce the amount of nitrogen in the steviol glycoside composition.

First claim

The invention claimed is:

1. A method for preparing a steviol glycoside composition, which method comprises:
providing a fermentation broth comprising a steviol glycoside;
subjecting the fermentation broth to a solid-liquid separation and a crystallization, to yield a first steviol glycoside composition;
combining the first steviol glycoside composition with water to form a steviol glycoside solution; and
recrystallizing, at pH 7.0 or above, a second steviol glycoside composition from the steviol glycoside solution, wherein:
the resulting second steviol glycoside composition comprises less nitrogen than the first steviol glycoside composition;
the nitrogen in the resulting second steviol glycoside composition is no more than 800 ppm; and
the resulting second steviol glycoside composition comprises at least 80% on a dry weight basis of at least one steviol glycoside selected from the group consisting of rebaudioside A, rebaudioside D, and rebaudioside M.

2. The method according to claim 1 , wherein the first steviol glycoside composition comprises at least 95% on a dry weight basis of at least one steviol glycoside.

3. The method according to claim 2 , wherein the crystallization is carried out at pH 8.0 or above.

4. The method according to claim 3 , wherein the resulting second steviol glycoside composition comprises nitrogen in an amount of no more than about 500 ppm.

5. The method according to claim 4 , wherein the resulting second steviol glycoside composition comprises nitrogen in an amount of no more than about 100 ppm.

6. The method according to claim 5 , wherein the resulting second steviol glycoside composition further comprises one or more of any one of rebaudioside B, rebaudioside C, rebaudioside E, rebaudioside F, stevioside, dulcoside A, rubusoside or steviolbioside.

7. The method according to claim 6 , wherein the resulting second steviol glycoside composition comprises at least 95% on a dry weight basis of at least one steviol glycoside.

8. The method according to claim 7 , wherein the first steviol glycoside composition comprises at least 80% on a dry weight basis of at least one steviol glycoside.

9. The method according to claim 7 , wherein the crystallization is carried out at pH 9.0 or above.

10. The method according to claim 7 , wherein the resulting second steviol glycoside composition comprises nitrogen in an amount of no more than about 200 ppm.

11. The method according to claim 7 , wherein the resulting second steviol glycoside composition comprises nitrogen in an amount of no more than about 50 ppm.

12. The method according to claim 7 , wherein the resulting second steviol glycoside composition comprises at least 97% on a dry weight basis of rebaudioside A.

13. The method according to claim 7 , wherein the resulting second steviol glycoside composition comprises at least 97% on a dry weight basis of rebaudioside D.

14. The method according to claim 7 , wherein the resulting second steviol glycoside composition comprises at least 97% on a dry weight basis of rebaudioside M.

Assignees

• Dsm Ip Assets Bv

Inventors

• Geertman Robert
• Galaev Igor

Classifications

• C12P19/44 Primary

  Preparation of O-glycosides, e.g. glucosides {(polysaccharides and not substituted disaccharides C12P19/04, C12P19/12)} · CPC title

• A23L2/60

  Sweeteners · CPC title

• A23L27/30 Primary

  Artificial sweetening agents · CPC title

• A23L33/20

  Reducing nutritive value; Dietetic products with reduced nutritive value · CPC title

• A23L27/36

  Terpene glycosides · CPC title