Method for producing psicose by using psicose epimerase producing microorganism
US-2020080072-A1 · Mar 12, 2020 · US
Microbacterium sp. strain and method for producing psicose by using same
US11066640B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11066640-B2 |
| Application number | US-201615775491-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 1, 2016 |
| Priority date | Dec 7, 2015 |
| Publication date | Jul 20, 2021 |
| Grant date | Jul 20, 2021 |
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Abstract
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The present invention relates to a newly isolated bacterium belonging to the genus Microbacterium, a composition for producing psicose comprising the strain, and a method for producing psicose using the same.
First claim
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The invention claimed is: 1. A method for producing psicose from fructose in the presence of manganese ion (Mn 2+ ), comprising: contacting the fructose with a microbial cell of Microbacterium foliorum having psicose conversion activity to produce psicose from fructose, a culture of Microbacterium foliorum , a culture supernatant of Microbacterium foliorum , a culture extract of Microbacterium foliorum , and/or a lysate of Microbacterium foliorum , wherein the Microbacterium foliorum has an increased psicose conversion activity in the presence of manganese ion (Mn 2+ ) compared to absence of manganese ion. 2. The method for producing the psicose of claim 1 , wherein the contacting comprises culturing the Microbacterium foliorum in a medium containing the fructose. 3. The method for producing the psicose of claim 1 , wherein the contacting is performed by contacting the fructose to a support immobilized with a microbial cell of Microbacterium foliorum , a culture of Microbacterium foliorum , a culture supernatant of Microbacterium foliorum , a culture extract of Microbacterium foliorum , and/or a lysate of Microbacterium foliorum. 4. The method for producing the psicose of claim 1 , wherein the method further comprises adding one or more kinds of metal ions selected from the group consisting of manganese and cobalt. 5. The method for producing the psicose of claim 1 , wherein the method is performed by not using a buffer solution. 6. The method for producing the psicose of claim 1 , wherein the fructose has a concentration of 40 to 75% (w/w). 7. The method for producing the psicose of claim 1 , wherein the method is performed under the condition of pH 6.5 to 9.0 and 40 to 80° C. 8. The method for producing the psicose of claim 1 , wherein the Microbacterium foliorum is deposited in Korean Culture Center of Microorganism under Accession Number KCCM11774P. 9. A composition for producing psicose from fructose, comprising a microbial cell of Microbacterium foliorum having psicose conversion activity to produce psicose from fructose, a culture of Microbacterium foliorum , a culture supernatant of Microbacterium foliorum , a culture extract of Microbacterium foliorum , and/or a lysate of Microbacterium foliorum , and manganese ion (Mn 2+ ), wherein the Microbacterium foliorum has an increased psicose conversion activity in the presence of manganese ion (Mn 2+ ), compared to absence of manganese ion. 10. The composition of claim 9 , wherein the Microbacterium foliorum has psicose conversion activity at 40 to 80° C. 11. The composition of claim 9 , wherein the Microbacterium foliorum has psicose conversion activity at pH 6.5 to 9.0. 12. The composition for producing the psicose of claim 9 , wherein the Microbacterium foliorum has 1.2 to 2.3 times increased psicose conversion activity in the presence of one or more kinds of metal ions selected from the group consisting of manganese and cobalt compared to absence of the metal ion. 13. The composition for producing the psicose of claim 9 , wherein the Microbacterium foliorum is deposited in Korean Culture Center of Microorganism under Accession Number KCCM11774P. 14. A Microbacterium foliorum deposited in Korean Culture Center of Microorganism under Accession Number KCCM11774P.
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- What does patent US11066640B2 cover?
- The present invention relates to a newly isolated bacterium belonging to the genus Microbacterium, a composition for producing psicose comprising the strain, and a method for producing psicose using the same.
- Who is the assignee on this patent?
- Samyang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12P19/02. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 20 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).