Endophytic microbial symbionts in plant prenatal care

Patent metadata

Field	Value
Publication number	US-11064673-B2
Application number	US-201816228640-A
Country	US
Kind code	B2
Filing date	Dec 20, 2018
Priority date	Feb 5, 2013
Publication date	Jul 20, 2021
Grant date	Jul 20, 2021

Abstract

The present disclosure provides novel endophyte strains or cultures thereof that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom.

First claim

The invention claimed is:

1. A plant seed manually or mechanically coated with at least one endophyte, wherein the plant is a cereal that is not wheat and the endophyte is a Streptomyces sp. strain or culture thereof which is deposited under IDAC 081111-06 or which comprises the 16S rDNA sequence as shown in SEQ ID NO:6.

2. The coated plant seed of claim 1 , wherein the plant is a barley plant.

3. A method of improving seed vitality, plant health and/or yield comprising cultivating the coated plant seed of claim 1 into a first generation plant.

4. The method of claim 3 , wherein the plant is a barley plant.

5. The method of claim 3 , wherein the improving seed vitality, plant health, and/or yield is for increasing seed germination, for decreasing time to reach the energy of germination, for reducing hydrothermal time required for germination, for increasing seed germination vigour, for increasing the fresh weight of seedlings, for enhancing Rhizobium nodulation traits, for increasing yield of seedlings, for reducing the effects of stress on the seed or cultivated plant, for reducing the effects of drought, heat and/or biotic stress on the seed or cultivated plant, for reducing a Fusarium infection on the seed or cultivated plant, for enhancing stratification, for breaking dormancy, for reducing the effects of stress by modulating gene expression of hormonal ent-kaurenoic (KAO), for repression of shoot growth (RSG), abscisic acid (ABA), gibberellic acid (GA), 14-3-3 genes and nitric oxide (NO) molecules, and/or for modulating gene expression of stress resistance superoxide dismutase (SOD), manganese SOD (MnSOD), proline (Pro) or MYB genes.

6. The method of claim 5 , wherein the plant is a barley plant.

7. A method of improving plant health and/or yield comprising manually or mechanically coating a plant seed with at least one endophyte, wherein the plant is a cereal that is not wheat and the endophyte is a Streptomyces sp. strain or culture thereof which is deposited under IDAC 081111-06 or which comprises the 16S rDNA sequence as shown in SEQ ID NO:6; and cultivating the coated plant seed into a first generation plant.

8. The method of claim 7 , wherein the plant is a barley plant.

9. The method of claim 7 , wherein the improving plant health and/or yield is for reducing the effects of stress.

10. The method of claim 9 , wherein the plant is a barley plant.

11. The method of claim 7 , wherein the improving plant health and/or yield is for reducing the effects of stress wherein the stress is heat, drought or biotic stress.

12. The method of claim 11 , wherein the plant is a barley plant.

13. A method of phytoremediation or phytoreclamation of a contaminated site comprising cultivating the coated plant seed of claim 1 on the site into a first generation plant; thereby remediating or reclaiming the site.

14. The method of claim 13 , wherein the plant is a barley plant.

15. The method of claim 13 , wherein the site is contaminated with an organic chemical, a salt or a metal.

16. The method of claim 13 , wherein the site is contaminated with an organic chemical and the organic chemical is a hydrocarbon or a petroleum.

17. The method of claim 13 , wherein the site is contaminated with metal and the metal is lead or cadmium and/or a radioisotope.

18. The plant seed of claim 1 , wherein the endophyte is the Streptomyces sp. strain or culture thereof which is deposited under IDAC 081111-06 and the plant is a barley plant.

19. A method of improving seed vitality, plant health and/or yield comprising cultivating the coated plant seed of claim 18 into a first generation plant.

20. The method of improving plant health and/or yield of claim 7 , wherein the plant seed is a barley plant seed and the endophyte is a Streptomyces sp. strain or culture thereof which is deposited under IDAC 081111-06.

Assignees

• Univ Saskatchewan

Inventors

• Vujanovic Vladimir
• Germida James J

Classifications

• A01N63/28

  Streptomyces · CPC title

• C12R2001/80

  Penicillium · CPC title

• A01H17/00 Primary

  Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza (lichens A01H15/00) · CPC title

• A01N63/30

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• A01H3/00

  Processes for modifying phenotypes {, e.g. symbiosis with bacteria} (A01H4/00 takes precedence) · CPC title