Charged mass labeling system

Patent metadata

Field	Value
Publication number	US-11061035-B2
Application number	US-201716071347-A
Country	US
Kind code	B2
Filing date	Jan 20, 2017
Priority date	Jan 22, 2016
Publication date	Jul 13, 2021
Grant date	Jul 13, 2021

Abstract

The invention generally relates to charged mass label compositions and methods of use thereof for detecting a target analyte in a sample. In certain aspects, the invention provides a charged mass label composition including an affinity reagent, and a mass label precursor bound to the affinity reagent. The mass label precursor includes a label binding unit and a mass label. The label binding unit reversibly binds the mass label to the affinity reagent. The mass label includes a charge unit and a mass label unit having a pre-defined mass-to-charge-value in a mass spectrum.

First claim

What is claimed is:

1. A charged mass label composition comprising:
an affinity reagent; and
a mass label precursor bound to the affinity reagent, wherein the mass label precursor comprises a label binding unit and a mass label, the label binding unit reversibly binding the mass label to the affinity reagent, wherein the mass label comprises a charge unit covalently coupled to a mass label unit through a fragmentation resistant covalent bond wherein the charge unit is capable of being released from the mass label upon ionization, wherein the mass label unit is neutral and has a pre-defined mass-to-charge-value in a mass spectrum, and wherein upon ionization, the charge unit releases from the mass label unit and the mass label unit is detectable in the mass spectrum due to the mass label unit having the pre-defined mass-to-charge-value in a mass spectrum;
wherein the charge unit comprises one or more chemical moieties selected from the group consisting of formulas

wherein at least one X is a quaternary ammonium cation or a quaternary phosphonium cation that is optionally substituted by at least one R selected from the group consisting of hydrogen, alkyl, and an aromatic organic derivative.

2. The charged mass label composition according to claim 1 , wherein the affinity reagent comprises at least one member of a specific binding pair.

3. The charged mass label composition according to claim 1 , wherein the charge unit is a chemical moiety selected from the group consisting of: carnitine, imidazolium, pyridinium, tetra ethyl amine, benzalkonium amine, alkyl benzethonium amine, triphenyl phosphonium cation, and trialkyl(2,3-dihydroxypropyl).

4. The charged mass label composition according to claim 1 , wherein the mass label unit comprises an arrangement of chemical moieties that produce one or more fragments upon ionization, wherein at least one of the one or more fragments has the pre-defined mass-to-charge-value in a mass spectrum.

5. The charged mass label composition according to claim 4 , wherein the pre-defined mass-to-charge-value in the mass spectrum of the mass label or one or more fragments of the mass label is different from background mass-to-charge-values in the mass spectrum.

6. The charged mass label composition according to claim 1 , wherein the label binding unit comprises a reversible bond.

7. The charged mass label composition according to claim 6 , wherein the reversible bond comprises an acetal or ketal bond.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Baird Zane
• Pugia Michael
• Hollerbach Adam
• Ayrton Stephen

Classifications

• G01N33/575

  for cancer · CPC title

• G01N33/57575

  involving oncogenic proteins · CPC title

• G01N2458/00

  Labels used in chemical analysis of biological material · CPC title

• G01N33/58 Primary

  involving labelled substances (G01N33/53 takes precedence) · CPC title

• G01N33/531

  Production of immunochemical test materials · CPC title