Biological sample treatment apparatus

Patent metadata

Field	Value
Publication number	US-11060957-B2
Application number	US-201816035848-A
Country	US
Kind code	B2
Filing date	Jul 16, 2018
Priority date	Nov 16, 2011
Publication date	Jul 13, 2021
Grant date	Jul 13, 2021

Abstract

Apparatus for treating biological samples disposed on substrates, including: an input buffer for receiving one or more substrate holders each being adapted to support a plurality of the substrates; a treatment zone including a plurality of treatment stations each being adapted to receive one of the substrates; a reagent dispenser configured by a controller to dispense reagents to the substrates at the treatment stations; a substrate transport device configured by the controller to transport individual substrates between the substrate holders in the input buffer and the treatment stations.

First claim

The invention claimed is:

1. An automated method for treating biological samples disposed on substrates, wherein the substrates are treated at treatment stations in a treatment zone, the method including the steps of:
receiving one or more of substrate holders in an input buffer, each substrate holder being adapted to support a plurality of the substrates;
using a substrate transport device, configured by a controller, to transport individual ones of the substrates between the substrate holders in the input buffer and the treatment stations; and
using a reagent dispenser, configured by the controller, to dispense reagents to the substrates at the treatment stations.

2. The method according to claim 1 , and further including the step of:
using the substrate transport device, configured by the controller, to transport the individual ones of the substrates between the treatment stations and the substrate holders in an output buffer for receiving the one or more of the substrate holders.

3. The method according to claim 1 , and further including the step of:
using the substrate transport device, configured by the controller, to transport the individual ones of the substrates into and out of a substrate covering unit for applying a cover member to the biological sample on the substrate.

4. The method according to claim 1 , and further including the steps of:
reading, using a substrate identification reader, a substrate identifier, borne by each of the substrates, when the substrate is the input buffer;
using the controller to access a database for storing a plurality of protocols, each protocol defining a sequence of treatment operations to be applied to one or more of the substrates; and
using the substrate transport device, configured by the controller, to group individual substrates within the treatment zone to efficiently treat the biological samples disposed on substrates.

5. The method according to claim 4 , and further including one or more of the following steps:
a. using the substrate transport device, configured by the controller, to group the individual ones of the substrates to which a common protocol is to be applied;
b. using the substrate transport device, configured by the controller, to group the individual ones of the substrates to which a common reagent is to be applied;
c. using the substrate transport device, configured by the controller, to group the individual ones of the substrates that share a common treatment time;
d. using the substrate transport device, configured by the controller, to transport the individual ones of the substrates so as to optimise throughput of the substrates during protocols and/or treatment operations; and
e. using the controller to maximise a number of protocols or treatment operations that can be consecutively performed on one or more of the substrates.

6. The method according to claim 5 , and further including the step of:
using the controller to optimise a number of reagent dispensing operations performed by the reagent dispenser to different substrates without changing reagents.

7. The method according to claim 4 , wherein the substrate identification reader is supported by the substrate transport device.

8. The method according to claim 1 , wherein the reagent dispenser is supported by the substrate transport device.

9. The method according to claim 1 , and further including the step of:
transferring reagent, of the reagents and using a reagent transfer system, from a plurality of reagent containers, housed below a work surface on which the treatment stations are disposed, to the reagent dispenser.

10. The method according to claim 9 , and further including the step of:
aspirating the reagent, using an aspiration probe of the reagent transfer system, from the reagent containers via a reagent transfer port of the reagent transfer system, wherein the reagent transfer port passes through the work surface.

11. The method according to claim 10 , and further including the step of:
selectively bringing, using a carousel of the reagent transfer system supporting the reagent containers, each reagent container into fluidic communication with the reagent transfer port.

12. The method according to claim 11 , and further including the step of:
loading and unloading the reagent containers from the carousel via a reagent container access port of the reagent transfer system.

13. The method according to claim 1 , and further including the step of:
accessing, by using the substrate transport device configured by the controller, the substrates from the one or more of the substrate holders disposed in a plurality of input compartments of the input buffer.

14. The method according to claim 13 , and further including the step of:
baking the substrates in the substrate holders disposed in one or more baking stations of one or more of the input compartments.

15. The method according to claim 13 , wherein the input compartments are adapted to support the substrate holders in a substantially horizontal orientation.

16. The method according to claim 2 , and further including the step of:
accessing, by using the substrate transport device configured by the controller, the substrates from the one or more of the substrate holders disposed in a plurality of output compartments of the output buffer.

17. The method according to claim 16 , and further including the step of:
curing the substrates in the substrate holders disposed in one or more curing stations of one or more of the output compartments.

18. The method according to claim 16 , wherein the output compartments are adapted to support the substrate holders in a substantially horizontal orientation.

19. The method according to claim 1 , wherein at least one of the substrate holders, received in the input buffer, supports a plurality of the substrates from a common patient case, and wherein the method further includes the step of:
using the controller to group the plurality of the substrates into at least the one of the substrate holders received in an output buffer according to that common patient case.

20. The method according to claim 1 , wherein the one or more of the substrate holders of the input buffer are ones of first substrate holders, and wherein the method further includes the steps of:
using the substrate transport device, configured by the controller, to individually transport one of the substrates from a first slot in one of the first substrate holders in the input buffer to one of the treatment stations; and
using the substrate transport device, configured by the controller, to individually transport the one of the substrates from the one of the treatment stations to a second slot in one of a plurality of second substrate holders in an output buffer.

21. The method according to claim 20 , wherein the substrate transport device includes a robotic arm including a scanner and a suction cup at an end of the robotic arm, and wherein the method further includes using the robotic arm, configured by the controller, to perform the following steps:
reach into the input buffer, scan, via the scanner attached to an end of the robotic arm, a label attached to one of the first substrate holders in a first label slot corresponding to the one of the substrates in the first slot;
grip, via the suction cup attached to the end of the robotic arm, the one of the substrates from the first slot of the one of the first substrate holders;
release the one of the substrates into one of the

Assignees

• Leica Biosystems Melbourne Pty

Inventors

• Dockrill Mark Brian
• Gason Samuel

Classifications

• G01N1/312 Primary

  for samples mounted on planar substrates · CPC title

• G01N35/00029

  provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence) · CPC title

• G01N2035/00079

  Evaporation covers for slides · CPC title

• G01N1/31 Primary

  Apparatus therefor · CPC title

• G01N2035/0094

  optimisation; experiment design · CPC title