Self-regenerating hyaluronan polymer brushes

Patent metadata

Field	Value
Publication number	US-11060074-B2
Application number	US-201916298820-A
Country	US
Kind code	B2
Filing date	Mar 11, 2019
Priority date	Mar 9, 2018
Publication date	Jul 13, 2021
Grant date	Jul 13, 2021

Abstract

The present invention provides polymer brush constructs, for example, surfaces decorated with hyaluronan polymers, and methods of making the same. The constructs are useful as functional materials for numerous applications, e.g., as lubricants, implant coatings, bandages and other uses.

First claim

What is claimed is:

1. A polymer brush comprising:
a substrate; and
hyaluronan synthase components attached to about 20 to about 40% of the surface area of the substrate, at least a portion of which being capable of synthesizing a hyaluronic acid having a certain hydrodynamic diameter;
wherein one or more of the hyaluronan synthase components comprise one or more hyaluronan synthase proteins in a fragment of cell membrane; and
wherein an average distance between the hyaluronan synthase proteins in the fragment of cell membrane is substantially equal to or less than an average hydrodynamic diameter of the hyaluronic acid.

2. The polymer brush of claim 1 , wherein an average distance between the hyaluronan synthase components on the substrate is less than about 1 μm.

3. The polymer brush of claim 1 , wherein the fragment of cell membrane comprises bacterial cell membrane.

4. The polymer brush of claim 1 , wherein the hyaluronan synthase proteins comprise a Streptococcus equisimilus (Group C) hyaluronan synthase.

5. The polymer brush of claim 1 , wherein the fragment of cell membrane comprises mammalian cell membrane.

6. The polymer brush of claim 1 , wherein the hyaluronic acid is an azide-modified hyaluronic acid.

7. The polymer brush of claim 1 , wherein an average height of the polymer brush is in the range of about 500 nm to about 30 μm.

8. The polymer brush of claim 1 , wherein an average size of the hyaluronan is less than about 12 MDa.

9. The polymer brush of claim 1 , wherein a grafting density of the polymer brush is modified such that it comprises regions comprising inactivated hyaluronan synthase components; and
wherein the inactivated hyaluronan synthase components are not capable of synthesizing polysaccharide.

10. The polymer brush of claim 1 further comprising hyaluronic acid polymers covalently attached to the substrate at certain grafting points on the substrate.

11. A polymer brush comprising:
a substrate; and
hyaluronan synthase components attached to the substrate, at least a portion of which being capable of synthesizing a hyaluronic acid having a certain hydrodynamic diameter;
wherein one or more of the hyaluronan synthase components comprise one or more hyaluronan synthase proteins in a fragment of cell membrane;
wherein an average distance between the hyaluronan synthase proteins in the fragment of cell membrane is substantially equal to or less than an average hydrodynamic diameter of the hyaluronic acid; and
wherein one or more of:
the hyaluronan synthase components comprise about 20 to about 40% of the surface area of the substrate;
an average distance between the hyaluronan synthase components on the substrate is less than about 1 μm;
the hyaluronic acid is an azide-modified hyaluronic acid;
an average height of the polymer brush is in the range of about 500 nm to about 30 μm;
an average size of the hyaluronan is less than about 12 MDa; and
a grafting density of the polymer brush is modified such that it comprises regions comprising inactivated hyaluronan synthase components that are not capable of synthesizing polysaccharide.

12. A method of fabricating the polymer brush of claim 11 comprising applying a solution comprising the hyaluronan synthase components to the substrate such that the hyaluronan synthase components are substantially immobilized on the substrate.

13. The method of claim 12 further comprising treating the substrate with a reaction mixture comprising a nucleotide sugar substrate.

14. The method of claim 13 , wherein the nucleotide sugar substrate comprises:
MgCl 2 ; and
at least one of:
uridine 5′-diphosphoglucuronic acid or a salt thereof; and
uridine 5′-diphospho-N-acetylglucosamine or a salt thereof.

15. The method of claim 13 , wherein treating the substrate provides a rapid polymerization.

16. The method of claim 15 , further comprising substantially disrupting the rapid polymerization with a treatment of a quenching mixture comprising about 30 mM to about 50 mM EDTA.

17. The method of claim 16 further comprising reinitiating the rapid polymerization by replacing the quenching mixture with a reaction mixture comprising a nucleotide sugar substrate.

18. The method of claim 15 , further comprising covalently binding the hyaluronic acid to the substrate to form a reinforced substrate comprising covalently-bound hyaluronic acid polymers.

19. A method of modifying the grafting density of the polymer brush of claim 11 comprising:
selecting a target region of the polymer brush having the hyaluronan synthase components; and
irradiating the target region with UV light such that the hyaluronan synthase components in the target region become inactivated;
wherein the inactivated hyaluronan synthase components are not capable of synthesizing polysaccharide.

Assignees

• Georgia Tech Res Inst

Inventors

• Curtis Jennifer E
• Finn M G
• Wei Wenbin
• Faubel Jessica

Classifications

• C12N11/16

  Enzymes or microbial cells immobilised on or in a biological cell · CPC title

• A01N25/10 Primary

  Macromolecular compounds · CPC title

• A61L31/10

  Macromolecular materials · CPC title

• A61L27/58

  Materials at least partially resorbable by the body · CPC title

• C12Y204/01212

  Hyaluronan synthase (2.4.1.212) · CPC title