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Immortalized sweat gland myoepithelial cell
US11060061B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11060061-B2 |
| Application number | US-201816626052-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 7, 2018 |
| Priority date | Aug 9, 2017 |
| Publication date | Jul 13, 2021 |
| Grant date | Jul 13, 2021 |
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Abstract
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An immortalized sweat gland myoepithelial cell which expresses α-SMA and pan-cytokeratin and has a sphere forming ability after subculture at least 5 times. A method for producing immortalized sweat gland myoepithelial said method comprising: while culturing a cell structure, wherein sweat gland myoepithelial cells are exposed on a surface, in a state of being suspended in a medium, transferring an immortalizing gene into the cells; and then culturing the transgenic structure thus obtained in a state of being suspended in the medium to thereby obtain immortalized sweat gland myoepithelial cells.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of producing an immortalized sweat gland myoepithelial cell, comprising the steps of: culturing a population of sweat gland cells in a suspended state in a medium, to form a sphere in which sweat gland myoepithelial cells are exposed on a surface of the sphere; introducing an immortalizing gene into the sweat gland myoepithelial cells present in the sphere while culturing the sphere in a suspended state in a medium to obtain a transgenic sphere; and culturing the transgenic sphere in a suspended state in a medium to obtain an immortalized sweat gland myoepithelial cell, wherein the immortalizing gene is an hTERT gene, a SV40T (large T) antigen gene, and a SV40t (small t) antigen gene. 2. The method of claim 1 , wherein the immortalizing gene is comprised in a viral vector.
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Classifications
Viral vectors · CPC title
Microcarriers · CPC title
Immortalised cells · CPC title
- C12N5/10Primary
Cells modified by introduction of foreign genetic material · CPC title
for animal cells · CPC title
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- What does patent US11060061B2 cover?
- An immortalized sweat gland myoepithelial cell which expresses α-SMA and pan-cytokeratin and has a sphere forming ability after subculture at least 5 times. A method for producing immortalized sweat gland myoepithelial said method comprising: while culturing a cell structure, wherein sweat gland myoepithelial cells are exposed on a surface, in a state of being suspended in a medium, transferrin…
- Who is the assignee on this patent?
- Mandom Corp, Univ Osaka
- What technology area does this patent fall under?
- Primary CPC classification C12N5/10. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 13 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).