Core-shell nanocomposite for metal-enhanced fluorescence

Patent metadata

Field	Value
Publication number	US-11060021-B2
Application number	US-201515542439-A
Country	US
Kind code	B2
Filing date	May 20, 2015
Priority date	May 20, 2014
Publication date	Jul 13, 2021
Grant date	Jul 13, 2021

Abstract

The present invention relates to a core-shell nanocomposite including fluorescent bodies disposed to have a uniform distance in a perpendicular direction from a surface thereof, to a method of manufacturing same, and to a use for a probe for metal-enhanced fluorescence.

First claim

The invention claimed is:

1. A fluorescent monomeric core-shell nanocomposite material comprising:
a nanoparticle of a first metal;
a plurality of linkers each having a uniform length bound to said first metal via one end thereof and having a fluorophore bound to the other end thereof, and
a shell of a second metal directly formed on said nanoparticle to which said linkers are bound,
wherein the shell comprises a thickness such that a surface of the shell is spaced apart a uniform distance from each of said fluorophores in a vertical direction,
wherein said plurality of fluorophores are coated with a silica shell, and
wherein said silica shell comprises a silica shell fluorophore that extends from said silica shell.

2. The core-shell nanocomposite material of claim 1 , wherein said nanoparticle of said first metal comprises a nanorod having an aspect ratio of 1.2 to 10.

3. The core-shell nanocomposite material of claim 1 , wherein said first metal and said second metal are independently selected from the group consisting of gold, silver, copper, palladium and platinum.

4. The core-shell nanocomposite material of claim 1 , wherein said first metal and said second metal have a lattice parameter ratio of 1:1.4 to 1.4:1.

5. The core-shell nanocomposite material of claim 1 , wherein said linkers are a single- or a double-stranded polynucleotide.

6. A probe for metal-enhanced fluorescence comprising a core-shell nanocomposite material of claim 1 .

7. The core-shell nanocomposite material of claim 1 , wherein said fluorophores on said plurality of linkers are identical.

8. The core-shell nanocomposite material of claim 1 , wherein said silica shell fluorophore and said plurality of fluorophores on said linkers are identical.

9. The core-shell nanocomposite material of claim 1 , wherein said nanocomposite material exhibits a metal-enhanced fluorescence effect.

10. A method of manufacturing a core-shell nanocomposite material comprising a plurality of fluorophores each disposed to have a uniform distance from a surface thereof in a vertical direction, said method comprising:
binding one end of each of a plurality of linkers having a uniform length onto a nanoparticle of a first metal, wherein the other end of said linker has a fluorophore bound thereof;
forming a shell of a second metal directly on said nanoparticle of said first metal to which the linkers are bound by adjusting a thickness of the shell of the second metal such that a surface of the shell of the second metal is [to be] spaced apart a uniform distance from the fluorophore; and
coating said core-shell nanocomposite material with a silica shell, wherein said silica shell comprises a silica shell fluorophore that extends from said silica shell.

11. The method of claim 10 , wherein said nanoparticle of said first metal comprises a nanorod having an aspect ratio of 1.2 to 10.

12. The method of claim 10 , wherein each of said first metal and said second metal is independently selected from the group consisting of gold, silver, copper, palladium and platinum.

13. The method of claim 10 , wherein said first metal and said second metal have a lattice parameter ratio of 1:1.4 to 1.4:1.

14. The method of claim 10 , wherein said linkers are a single- or a double-stranded polynucleotide.

15. The method of claim 10 , wherein said fluorophores on said plurality of linkers are identical.

16. The method of claim 10 , wherein said silica shell fluorophore and said plurality of fluorophores on said linkers are identical.

Assignees

• Snu R&Db Foundation

Inventors

• Nam Jwa Min
• Hwang Jae Ho
• Kim Min Ho
• Lee Jung Hoon

Classifications

• C09K11/58

  containing copper, silver or gold · CPC title

• C09K11/02

  Use of particular materials as binders, particle coatings or suspension media therefor · CPC title

• G01N21/648

  using evanescent coupling or surface plasmon coupling for the excitation of fluorescence · CPC title

• C09K11/025 Primary

  non-luminescent particle coatings or suspension media · CPC title

• G01N33/582

  with fluorescent label · CPC title