Magnetic capture of a target from a fluid

Patent metadata

Field	Value
Publication number	US-11059050-B2
Application number	US-201916414168-A
Country	US
Kind code	B2
Filing date	May 16, 2019
Priority date	Oct 27, 2014
Publication date	Jul 13, 2021
Grant date	Jul 13, 2021

Abstract

Disclosed herein is an improved method for magnetic capture of target molecules (e.g., microbes) in a fluid. Kits and solid substrates for carrying the method described herein are also provided. In some embodiments, the methods, kits, and solid substrates described herein are optimized for separation and/or detection of microbes and microbe-associated molecular pattern (MAMP) (including, e.g., but not limited to, a cell component of microbes, lipopolysaccharides (LPS), and/or endotoxin).

First claim

What is claimed is:

1. A device comprising a magnetic separation chamber, wherein at least a portion of a fluid-contact surface of the magnetic separation chamber comprises magnetic field gradient concentrating particles distributed thereon, and wherein magnetic field gradient concentrating particles are substantially aligned along magnetic flux lines of the magnetic field in presence of a magnetic field gradient, and wherein the magnetic field gradient concentrating particles act as local magnetic field gradient concentrators in presence of the magnetic field gradient.

2. A kit comprising (i) a device according to claim 1 ; (ii) one or more containers containing magnetic field gradient concentrating particles; and (iii) one or more containers containing target-binding magnetic particles.

3. The device of claim 1 , wherein the magnetic field gradient concentrating particles form magnetic micro- or nano-structures on said at least a portion of the fluid-contact surface of the magnetic separation chamber.

4. The device of claim 1 , wherein at least 50% area or higher of said at least a portion of the fluid-contact surface comprises the magnetic field gradient concentrating particles distributed thereon.

5. The device of claim 1 , wherein the magnetic separation chamber comprises a channel, a microfluidic channel, a sample well, a microtiter plate, a slide, a flask, a tube, a nanotube, a fiber, a filter, a membrane, a scaffold, an extracorporeal device, a mixer, a hollow fiber, or any combinations thereof.

6. The device of claim 1 , wherein the magnetic separation chamber is configured to remove target-binding magnetic particles from a fluid flowing through the magnetic separation chamber.

7. The device of claim 6 , wherein the target-binding magnetic particles are magnetic particles adapted to bind a target in the fluid.

8. The device of claim 7 , wherein the target-binding magnetic particles comprise on their surface microbe-binding molecules.

9. The device of claim 6 , wherein the target-binding magnetic particles are paramagnetic particles adapted to bind a target in the fluid.

10. The device of claim 1 , wherein the magnetic field gradient concentrating particles have a size from about 50 nm to about 5 mm.

11. The device of claim 1 , wherein the magnetic field gradient concentrating particles are a mixture of different sized magnetic field gradient concentrating particles.

12. The device of claim 1 , wherein the magnetic separation chamber comprises a source fluid channel and a collection fluid channel connected to each other by a plurality of transfer channels.

13. The device of claim 12 , wherein the plurality of transfer channels is oriented substantially perpendicular to the source channel and the collection channel.

14. The device of claim 13 , wherein the device further comprises a first magnetic source positioned in close proximity to the collection channel.

15. The device of claim 14 , wherein the device further comprises a second magnetic source positioned in close proximity to the source channel.

16. The device of claim 12 , wherein the source fluid channel is connected to a first fluid source.

17. The device of claim 12 , wherein the collection fluid channel is connected to a fluid source.

18. A solid substrate comprising (i) a surface having magnetic field gradient concentrating particles distributed thereon and substantially aligned along magnetic flux lines of a magnetic field, and wherein the magnetic field gradient concentrating particles act as local magnetic field gradient concentrators in the presence of the magnetic field gradient; (ii) target-binding magnetic particles; and (iii) a target.

Assignees

• Harvard College

Inventors

• Kang Joo Hun
• Ingber Donald E
• Super Michael

Classifications

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• B03C1/288

  disposed at the outer circumference of a recipient · CPC title

• B03C1/286

  disposed at the inner circumference of a recipient, e.g. magnetic drain bolt · CPC title

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• G01N33/54366

  Apparatus specially adapted for solid-phase testing · CPC title