Preparation method of water-soluble phytosterol nano-dispersion system with intestinal targeted release function

Patent metadata

Field	Value
Publication number	US-11058693-B1
Application number	US-202017001241-A
Country	US
Kind code	B1
Filing date	Aug 24, 2020
Priority date	Mar 24, 2020
Publication date	Jul 13, 2021
Grant date	Jul 13, 2021

Abstract

The invention discloses a method for preparing a water-soluble phytosterol nano-dispersion system with intestinal targeted release function, which comprises the following steps: preparing a phytosterol solution; preparing an ethanol aqueous solution containing pectin; then adding zein to obtain a zein pectin solution; mixing the phytosterol solution and the zein pectin solution by means of ultrasonic treatment to obtain a nanoparticle dispersion liquid; rotavaping the nano-particle dispersion liquid to remove ethanol and water to obtain a zein/pectin loaded phytosterol nano-dispersion system. In this invention, not only an ultrasonic method and an anti-solvent method are adopted, but also a pectin component is added with zein as an entrapping agent, which ensures an effective entrapping of phytosterol by zein while further facilitating to form a more compact interface structure and improving a stability of the nano-dispersion system.

First claim

The invention claimed is:

1. A method for preparing a water-soluble phytosterol nano-dispersion system with intestinal targeted release function, wherein the phytosterol is stigmasterol, wherein the method comprises the following steps:
(1) dissolving the stigmasterol in absolute ethanol to obtain a stigmasterol solution;
(2) pre-dissolving pectin in deionized water, and then adding with ethanol to obtain an ethanol aqueous solution containing pectin; adding zein into the ethanol aqueous solution to obtain a zein pectin solution, wherein a mass ratio of pectin to zein is 1:10;
(3) mixing the stigmasterol solution in the step (1) and the zein pectin solution in the step (2) by means of ultrasonic treatment to obtain a nanoparticle dispersion liquid;
(4) rotavaping the nanoparticle dispersion liquid to remove the organic solvent and water to obtain a water-soluble stigmasterol nano-dispersion system.

2. The method for preparing a water-soluble phytosterol nano-dispersion system with intestinal targeted release function according to claim 1 , wherein the ethanol aqueous solution in step (2) contains 80-90% ethanol by volume.

3. The method for preparing a water-soluble phytosterol nano-dispersion system with intestinal targeted function according to claim 1 , wherein in the step (3), the ultrasonic treatment conditions are as follows: a continuous ultrasonic treatment using a probe ultrasonic processor with an ultrasonic frequency of 15-25 kHz, a power of 150-250 W, and a lasted time of 1-3 min.

4. The method for preparing a water-soluble phytosterol nano-dispersion system with intestinal targeted function according to claim 3 , wherein after the continuous ultrasonic treatment is finished, the ultrasonic treatment is continued with a pulse mode, with a pulse frequency of 1 pulse per second and a lasted time of 0.5˜1.5 min.

5. The method for preparing a water-soluble phytosterol nano-dispersion system with intestinal targeted function according to claim 1 , wherein in the nanoparticle dispersion liquid in the step (3), a mass ratio of stigmasterol, pectin and zein is 1:0.5:5.

6. The method for preparing a water-soluble phytosterol nano-dispersion system with intestinal targeted function according to claim 1 , wherein in the step (4), a temperature for the rotavaping is 40-50° C., with a rotating speed of 50-60 rpm.

Assignees

• Zhengjiang Univ Of Technology
• Univ Zhejiang Technology

Inventors

• Feng Simin
• Sun Peilong
• Shao Ping
• Yan Jiadan
• Wang Dan
• Sun Yuxin

Classifications

• A61K47/36

  Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin · CPC title

• A61K9/5169

  Proteins, e.g. albumin, gelatin · CPC title

• A61K9/10

  Dispersions; Emulsions · CPC title

• A61K31/575 Primary

  substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol · CPC title

• A23L33/11

  Plant sterols or derivatives thereof, e.g. phytosterols · CPC title