Systems and methods for monitoring and controlling drosophila activity

Patent metadata

Field	Value
Publication number	US-11058093-B2
Application number	US-201615771844-A
Country	US
Kind code	B2
Filing date	Oct 31, 2016
Priority date	Oct 30, 2015
Publication date	Jul 13, 2021
Grant date	Jul 13, 2021

Abstract

Systems and methods for monitoring and controlling activities of Drosophila organisms are provided. In one aspect, a method includes acquiring, using a first activity detector, imaging data tracking movements of the Drosophila organisms, and acquiring, using a second activity detector, bioluminescence data corresponding to a neural activity of the Drosophila organisms. The method also includes correlating, using the acquired data, a behavioral activity and neural activity of the Drosophila organisms, and determining, using the correlation, an activity profile for the Drosophila organisms. The method further includes providing, based on the activity profile, a stimulation to the Drosophila organisms to control at least one of the behavior activity or the neural activity over a time period extendible to a nominal life cycle of the Drosophila organisms.

First claim

The invention claimed is:

1. A system for monitoring activities of Drosophila organisms, the system comprising:
an organism holder comprising a plurality of chambers configured for holding Drosophila organisms therein, the Drosophila organisms having a neuron co-expressing a channelrhodopsin and a calcium-dependent transcription activator configured to express luciferase;
a first activity detector configured to acquire imaging data tracking movements of the Drosophila organisms;
a second activity detector configured to acquire bioluminescence data corresponding to a neural activity of the Drosophila organisms;
an activity control module configured to apply optogenetic stimulation to the Drosophila with a light source;
a light-tight enclosure having a light-tight vent, the light-tight enclosure including the organism holder, the first activity detector, the activity control module, and the second activity detector;
a processor configured to:
provide optogenetic stimulation to the neuron using the light source at an intensity and duration sufficient to modify bioluminescence intensity relative to the absence of optogenetic stimulation;
receive imaging data and bioluminescence data acquired over a time period extendible to a nominal life cycle of the Drosophila organisms;
correlate, using the received data, changes in a behavioral activity and the bioluminescence data as a result of the applied optogenetic stimulation of the Drosophila organisms;
determine, based on the correlation, an activity profile for the Drosophila organisms, the activity profile including information associated with a sleep pattern or a wake pattern of the Drosophila organisms;
generate, using the activity profile, a report indicating a relationship between the neuron and the sleep pattern or the wake pattern of the Drosophila organism; and

an output for displaying the report.

2. The system of claim 1 , wherein the plurality of chambers are configured to include a sufficient amount of nourishment for sustaining the Drosophila organisms over the time period.

3. The system of claim 1 , wherein the time period is extendible to 4 weeks.

4. The system of claim 1 , wherein the activity control module further includes one or more of a vibration source, a temperature source, an electrical source, or a combination thereof, configured to apply the stimulation.

5. The system of claim 1 , wherein the one or more light source includes a light emitting diode.

6. The system of claim 1 , wherein the organism holder comprises multiple chamber units that can be assembled together.

7. The system of claim 1 , wherein the organism holder comprises a 96-well plate.

8. The system of claim 1 , wherein the calcium-dependent transcription activator configured to express luciferase comprises CaLexA-LUC.

9. The system of claim 1 , wherein the channelrhodopsin comprises CsChrimson.

10. A method for controlling activities of Drosophila organisms, the method comprising:
acquiring, using a first activity detector, imaging data tracking movements of the Drosophila organisms from within an organism holder comprising a plurality of chambers configured for holding the Drosophila organisms therein, the Drosophila organisms having a neuron co-expressing a channelrhodopsin and a calcium-dependent transcription activator configured to express luciferase;
acquiring, using a second activity detector, bioluminescence data corresponding to a neural activity of the Drosophila organisms;
providing, using a light source, optogenetic stimulation to the neuron at an intensity and duration sufficient to modify bioluminescence in the neuron;
correlating, using the acquired data, changes in a behavioral activity and the bioluminescence data as a result of the applied optogenetic stimulation of the Drosophila organisms;
determining, using the correlation, an activity profile for the Drosophila organisms, the activity profile including information associated with a sleep pattern or a wake pattern of the Drosophila organisms; and
generating, using the activity profile, a report indicating a relationship between the neuron and the sleep pattern or the wake pattern.

11. The method of claim 10 , wherein the method further comprises providing an additional stimulation using one or more of a light source, a temperature source, a vibration source, an electrical source, or a combination thereof.

12. The method of claim 10 , wherein the method further comprises generating, using the activity profile, information associated with a circadian cycle of the Drosophila organisms.

13. The method of claim 10 wherein the time period is extendible to 4 weeks.

14. A system for monitoring activities of Drosophila organisms, the system comprising:
an organism holder comprising a plurality of chambers configured for holding Drosophila organisms therein;
a vibration source mechanically coupled to the organism holder, the vibration source configured to induce or transmit vibrations to the organism holder;
a first activity detector configured to acquire imaging data tracking movements of the Drosophila organisms;
a second activity detector configured to acquire bioluminescence data corresponding to a neural activity of the Drosophila organisms;
a processor configured to:
receive imaging data and bioluminescence data acquired over a time period extendible to a nominal life cycle of the Drosophila organisms;
correlate, using the received data, a behavioral activity and a neural activity of the Drosophila organisms;
determine, based on the correlation, an activity profile for the Drosophila organisms;
generate, using the activity profile, a report indicative of a condition of the Drosophila organisms over the time period;

an output for displaying the report, and
wherein the organism holder rests on a base, and wherein the vibration source moves the organism holder along a track defined by guides on the base.

15. The system of claim 14 , wherein the vibration source is a solenoid.

16. The system of claim 14 , wherein the organism holder rests on a base elevated by vibration isolation supports.

Assignees

• Univ Brandeis

Inventors

• Guo Fang
• Jung Hyung-Jae
• Rosbash Michael

Classifications

• A01K1/0047

  Air-conditioning, e.g. ventilation, of animal housings (Laboratory cage ventilation A01K1/0313; Cat or dog houses with climate control means A01K1/0335) · CPC title

• A01K29/005

  Monitoring or measuring activity · CPC title

• A01K1/031 Primary

  Cages for laboratory animals; Cages for measuring metabolism of animals · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• A01K67/033

  Human Necessities · mapped topic