Two-chamber dual-pore device

Patent metadata

Field	Value
Publication number	US-11054390-B2
Application number	US-201916443025-A
Country	US
Kind code	B2
Filing date	Jun 17, 2019
Priority date	Nov 14, 2011
Publication date	Jul 6, 2021
Grant date	Jul 6, 2021

Abstract

Provided is a device comprising a channel through and defined by a plurality of layers surrounding the channel, the channel connecting a first and a second chambers separated by the plurality of layers, wherein the plurality of layers comprise a first layer, a second layer; and a conductive middle layer disposed between the first and second layers, wherein the channel comprises (a) a first region defined by the first layer, denoted as an inlet, that is about 0.5 nm to about 100 nm in diameter and (b) a second region defined by the second layer, denoted as an outlet, wherein the inlet and the outlet are about 10 nm to about 1000 nm apart from each other, and wherein the first and second chambers and the middle layer are connected to a power supply. Also provided are methods of preparing and using the device, in particular for nucleic acid sequencing.

First claim

The invention claimed is:

1. A system comprising:
a channel through and defined by a plurality of layers surrounding the channel, the channel connecting a first chamber and a second chamber separated by the plurality of layers,
the plurality of layers comprising:
a first layer, and
a conductive layer coupled to the first layer and surrounding the channel,

the channel comprising an inlet and an outlet defined by the plurality of layers, the inlet and the outlet being between 10 nm and 1000 nm apart from each other,
the conductive layer coupled to a power supply,
wherein the system further comprises at least one sensor capable of detecting a polymer component during movement of the polymer component into the inlet, and
wherein the sensor measures an ionic current through the inlet.

2. The system of claim 1 , wherein at least one of the inlet and the outlet defines an opening that is from 0.5 nm to 100 nm in diameter.

3. The system of claim 1 , wherein at least one of the inlet and the outlet is defined by a layer having a thickness of from 0.1 nm to 100 nm.

4. The system of claim 1 , further comprising a first electrode coupled to the conductive layer.

5. The system of claim 4 , further comprising a second electrode positioned within the first chamber.

6. The system of claim 1 , wherein the conductive layer is composed of at least one of gold, platinum, aluminum, and chrome.

7. The system of claim 1 , wherein the power supply is configured to provide a first voltage V 1 between the first chamber and the conductive layer, and a second voltage V 2 between the conductive layer and the second chamber.

8. The system of claim 7 , wherein the first voltage V 1 and the second voltage V 2 are independently adjustable.

9. The system of claim 1 , wherein the second chamber is coupled to a fluid channel in fluid communication with a reservoir of the system.

10. The system of claim 1 , wherein the inlet is coaxial with the outlet.

11. The system of claim 1 , wherein the polymer component comprises a nucleotide, and wherein the system is configured to translocate the nucleotide from the inlet to the outlet with application of a voltage differential across the first chamber and the second chamber by the power supply.

12. The system of claim 1 , wherein the polymer component comprises a double stranded DNA component.

13. The system of claim 1 , wherein the polymer component comprises a single stranded nucleic acid component.

14. A method for controlling movement of a charged polymer through a channel, the method comprising:
receiving a sample comprising a charged polymer into one of a first chamber and a second chamber of a system, the system comprising:
a channel through and defined by a plurality of layers surrounding the channel, the channel fluidly coupling the first chamber and the second chamber,
the plurality of layers comprising:
a first layer, and
a conductive layer coupled to the first layer,

wherein the channel comprises an inlet and an outlet defined by the plurality of layers, and
wherein the inlet and the outlet are between 10 nm and 1000 nm apart from each other; and
applying a differential voltage across the first chamber and the second chamber,
wherein applying the differential voltage comprises setting a first voltage V 1 at the first chamber and a second voltage V 2 at the second chamber, and
wherein the first voltage V 1 is different in magnitude from the second voltage V 2 , thereby controllably driving the charged polymer through the channel in one direction.

15. The method of claim 14 , further comprising adjusting the first voltage V 1 and the second voltage V 2 such that the first voltage V 1 and the second voltage V 2 generate forces to pull the charged polymer away from the conductive layer.

16. The method of claim 14 , wherein the first voltage and second voltage are 10 to 10,000 times greater in magnitude than the difference between the first voltage V 1 and the second voltage V 2 .

17. The method of claim 14 , wherein the charged polymer comprises one of: a double stranded DNA component and a single stranded nucleic acid component.

Assignees

• Univ California
• Univ Brigham Young

Inventors

• Dunbar William
• Schmidt Holger
• Hawkins Aaron

Classifications

• G01N33/48721 Primary

  Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68) · CPC title

• C12Q1/6869

  Methods for sequencing · CPC title

• G01N27/44791 Primary

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• C12Q2565/631

  being a biochannel or pore · CPC title