Plant regulatory elements and uses thereof

Patent metadata

Field	Value
Publication number	US-11053510-B2
Application number	US-201916563801-A
Country	US
Kind code	B2
Filing date	Sep 6, 2019
Priority date	Mar 11, 2016
Publication date	Jul 6, 2021
Grant date	Jul 6, 2021

Abstract

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

First claim

What is claimed is:

1. A recombinant DNA molecule comprising a DNA sequence polynucleotide selected from the group consisting of:
a) a polynucleotide with at least 95 percent sequence identity to SEQ ID NO: 8, wherein the polynucleotide has promoter activity;
b) a polynucleotide with at least 97 percent sequence identity to SEQ ID NO: 7, wherein the polynucleotide sequence has gene-regulatory activity;
c) a polynucleotide comprising any of SEQ ID NO: 7 or 8; and
d) a fragment comprising at least 150 contiguous nucleotides of SEQ ID NO: 8, wherein the fragment has promoter activity;

wherein said DNA molecule is operably linked to a heterologous transcribable DNA molecule.

2. The recombinant DNA molecule of claim 1 , wherein said polynucleotide has at least 97 percent sequence identity to the DNA sequence of SEQ ID NO: 8.

3. The recombinant DNA molecule of claim 1 , wherein said polynucleotide has at least 99 percent sequence identity to the DNA sequence of SEQ ID NO: 7 or 8.

4. The recombinant DNA molecule of claim 3 , wherein said polynucleotide comprises the DNA sequence of SEQ ID NO: 7 or 8.

5. The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable DNA molecule comprises a gene of agronomic interest.

6. The recombinant DNA molecule of claim 5 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

7. The recombinant DNA molecule of claim 5 , wherein the gene of agronomic interest confers pest resistance in plants.

8. A transgenic plant cell comprising a recombinant DNA molecule comprising a polynucleotide selected from the group consisting of:
a) a polynucleotide with at least 95 percent sequence identity to SEQ ID NO: 8, wherein the polynucleotide has promoter activity;
b) a polynucleotide with at least 97 percent sequence identity to SEQ ID NO: 7, wherein the polynucleotide sequence has gene-regulatory activity;
c) a polynucleotide comprising SEQ ID NO: 7 or 8; and
d) a fragment comprising at least 150 contiguous nucleotides of SEQ ID NO: 8, wherein the fragment has promoter activity;

wherein said DNA molecule is operably linked to a heterologous transcribable DNA molecule.

9. The transgenic plant cell of claim 8 , wherein said transgenic plant cell is a monocotyledonous plant cell.

10. The transgenic plant cell of claim 8 , wherein said transgenic plant cell is a dicotyledonous plant cell.

11. A transgenic plant, or part thereof, comprising the recombinant DNA molecule of claim 1 .

12. A progeny plant of the transgenic plant of claim 11 , or a part thereof, wherein the progeny plant or part thereof comprises said recombinant DNA molecule.

13. A transgenic seed, wherein the seed comprises the recombinant DNA molecule of claim 1 .

14. A method of producing a commodity product comprising obtaining the transgenic plant or part thereof according to claim 11 and producing the commodity product therefrom.

15. The method of claim 14 , wherein the commodity product is protein concentrate, protein isolate, grain, starch, seeds, meal, flour, biomass, or seed oil.

16. A method of expressing a transcribable DNA molecule comprising obtaining the transgenic plant according to claim 11 and cultivating the plant, wherein the transcribable DNA is expressed.

Assignees

• Monsanto Technology Llc

Inventors

• Chittoor Jaishree M
• Flasinski Stanislaw
• Oufattole Mohammed
• Petersen Michael W

Classifications

• C12N15/8222

  Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8274

  for herbicide resistance · CPC title