Simple LAMP PCR design for low resource setting and minimal environmental footprint

Patent metadata

Field	Value
Publication number	US-11052330-B2
Application number	US-201715476753-A
Country	US
Kind code	B2
Filing date	Mar 31, 2017
Priority date	Mar 31, 2016
Publication date	Jul 6, 2021
Grant date	Jul 6, 2021

Abstract

The present invention provides a device and method for testing a material for the presence of DNA. The system includes a centrifuge, a microchip performing cell lysis and an enclosure that contains an isothermal ballast material and chromogenic agent that melts at a specific temperature and displays a color change, respectively.

First claim

What is claimed is:

1. A method for testing a material comprising the steps of:
obtaining a sample of solution of cells forming a first analyte and placing said sample in an enclosure;
concentrating said cells in said first analyte;
lysing said cells;
extracting DNA from said cells to form a second analyte;
applying heat at predetermined temperature to said enclosure to amplify said DNA in said second analyte;
using a phase change material that melts at a predetermined temperature to maintain said predetermined temperature during said heating; and
detecting said DNA.

2. The method of claim 1 wherein said material is a wax that includes a thermochromic pigment that changes color at temperature.

3. The method of claim 1 further including a heating element.

4. The method of claim 3 wherein the heating element is powered by a battery.

5. The method of claim 3 wherein the heating element is powered by a rechargeable battery.

6. The method of claim 1 wherein said cells are concentrated in said first analyte by locating said first analyte in one or more containers, affixing said one or more containers to a bicycle wheel, and spinning said wheel.

7. The method of claim 1 further including the step of heating said second analyte by chemical heating.

8. The method of claim 1 wherein said visualization is performed by staining DNA in said second analyte with a dye.

9. The method of claim 8 wherein said dyeing is performed using an SYBR green technique.

10. The method of claim 1 wherein DNA extraction is performed by adsorption to silica.

11. The method of claim 10 wherein DNA adsorbed by silica in the presence of chaotropic salt solutions.

12. A method for testing a material for the presence of DNA without the use of an electric current comprising the steps of:
obtaining a sample of solution of cells to form a first analyte;
concentrating said cells in said first analyte;
lysing said cells;
extracting DNA from said cells to form a second analyte, said second analyte is heated at a predetermined temeperture in an enclosure lined with a phase change material that melts at a predetermined temperature to maintain said predetermined temperature during heating and includes a thermochromic pigment that changes color at a temperature equal to or greater than said predetermined temperature;

detecting said DNA.

13. The method of claim 12 wherein said cells are concentrated in said first analyte by locating said first analyte in one or more containers, affixing said one or more containers to a bicycle wheel, and spinning said wheel.

14. The method of claim 12 further including the step of heating said second analyte by chemical heating.

15. The method of claim 12 wherein said visualization is performed by staining DNA in said second analyte with a dye.

16. The method of claim 15 wherein said dyeing is performed using an SYBR green technique.

17. The method of claim 12 wherein DNA extraction is performed by adsorption to silica.

18. The method of claim 12 wherein DNA adsorbed by silica in the presence of chaotropic salt solutions.

19. The method of claim 12 , wherein said DNA in said second analyte are enclosed by said phase change material.

20. The method of claim 1 wherein said enclosure is lined with said phase change material.

Assignees

• Univ Arkansas
• NowDiagnostics
• Nowdiagnostics Inc

Inventors

• Beitle Robert
• Hestekin Christa N
• Elmasheiti Ahmed
• Cribbs Kimberly
• Rienisch Michael
• Jones Bryce Cameron
• Swearingen Allysa
• Hart Brandon
• Clark Kevin
• Thompson Vicki

Classifications

• G01N31/22

  using chemical indicators (G01N31/02 takes precedence) · CPC title

• B01L2200/10

  Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept · CPC title

• B01L2300/0627

  Sensor or part of a sensor is integrated · CPC title

• B01L7/00

  Heating or cooling apparatus (autoclaves B01J3/04); Heat insulating devices · CPC title

• B01L2200/147

  Employing temperature sensors · CPC title