Mass spectral tissue analysis

Patent metadata

Field	Value
Publication number	US-11047869-B2
Application number	US-201815891793-A
Country	US
Kind code	B2
Filing date	Feb 8, 2018
Priority date	May 18, 2011
Publication date	Jun 29, 2021
Grant date	Jun 29, 2021

Abstract

The invention generally relates to mass spectral analysis. In certain embodiments, methods of the invention involve analyzing a tissue sample using a mass spectrometry technique, in which the technique utilizes a liquid phase that does not destroy native tissue morphology during analysis. Due to the use of a liquid phase that does not destroy native tissue morphology during analysis, a subsequent staining technique can be performed on the tissue sample and an overlaid image can be produced of a mass spectral image and a staining image.

First claim

What is claimed is:

1. A method for producing an overlaid image of a tissue sample, the method comprising:
receiving mass spectral data of a tissue sample that has been generated by using a desorption electrospray ionization (DESI) technique that comprises directing a liquid phase that does not destroy native tissue morphology from a DESI probe onto the tissue sample to thereby desorb one or more compounds from the tissue sample that are analyzed by a mass spectrometer to produce the mass spectral data of the tissue sample, wherein the liquid phase is at least one selected from the group consisting of: dimethylformamide (DMF); tetrahydrofuran (THF); DMF comprising at least one other component DMF:ethanol (EtOH); DMF:water (H 2 O); DMF:acetonitrile (ACN); methanol (MeOH):chloroform (CHCl 3 ); ACN:CHCl 3 ; and ACN:EtOH;
producing a mass spectral image of the tissue sample based on the mass spectral data;
receiving histochemical data of the tissue sample;
producing an optical image of the tissue sample from the histochemical data; and
overlaying the mass spectral image of the tissue sample with the optical image of the tissue sample to produce an overlaid image.

2. The method according to claim 1 , further comprising determining a distribution of the one or more compounds in the tissue sample based on an analysis of the overlaid image.

3. The method according to claim 1 , wherein the histochemical data is produced by H&E staining.

4. The method according to claim 1 , wherein the one or more compounds comprises one or more lipids.

5. The method according to claim 2 , further comprising determining whether an abnormality exists in the tissue sample based on the distribution of the one or more compounds in the tissue sample.

6. The method according to claim 5 , wherein the abnormality is a cancer.

7. A method for producing an overlaid image of a tissue sample comprising a cancerous section, the method comprising:
receiving mass spectral data of a tissue sample comprising a cancerous section that has been generated by using a desorption electrospray ionization (DESI) technique that comprises directing a liquid phase that does not destroy native tissue morphology from a DESI probe onto the tissue sample to thereby desorb one or more compounds from the tissue sample that are analyzed by a mass spectrometer to produce the mass spectral data of the tissue sample, wherein the liquid phase is at least one selected from the group consisting of: dimethylformamide (DMF); tetrahydrofuran (THF); DMF comprising at least one other component DMF:ethanol (EtOH); DMF:water (H 2 O); DMF:acetonitrile (ACN); methanol (MeOH):chloroform (CHCl 3 ); ACN:CHCl 3 ; and ACN:EtOH;
producing a mass spectral image of the tissue sample comprising the cancerous section based on the mass spectral data;
receiving histochemical data of the tissue sample comprising the cancerous section;
producing an optical image of the tissue sample comprising the cancerous section from the histochemical data; and
overlaying the mass spectral image of the tissue sample comprising the cancerous section with the optical image of the tissue sample comprising the cancerous section to produce an overlaid image.

8. The method according to claim 7 , further comprising identifying the cancerous section within the overlaid image.

9. The method according to claim 7 , wherein the histochemical data is produced by H&E staining.

10. The method according to claim 7 , wherein the one or more compounds comprises one or more lipids.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Eberlin Livia Schiavinato
• Ferreira Christina Ramires
• Dill Allison Lisa
• Ifa Demian R

Classifications

• H01J49/0004

  Imaging particle spectrometry · CPC title

• G01N33/92 Primary

  involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)} · CPC title

• H01J49/165

  Electrospray ionisation · CPC title

• H01J49/142

  using a solid target which is not previously vapourised · CPC title

• H01J49/0036

  Step by step routines describing the handling of the data generated during a measurement · CPC title