14-hydroxy-docosahexaenoic acid compounds
US-9611238-B2 · Apr 4, 2017 · US
Method for producing rare fatty acid using novel enzyme, and novel rare fatty acid
US11046980B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11046980-B2 |
| Application number | US-201916404241-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 6, 2019 |
| Priority date | Jan 24, 2014 |
| Publication date | Jun 29, 2021 |
| Grant date | Jun 29, 2021 |
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Abstract
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The present invention provides production of hydroxylated fatty acid by a hydration reaction using a novel enzyme derived from Lactobacillus and using fatty acid as a substrate, and further, a production method of oxo fatty acid by an enzyme reaction or chemical oxidation reaction using the hydroxylated fatty acid as a substrate. In addition, a valuable novel rare fatty acid obtained by such production method is also provided.
First claim
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The invention claimed is: 1. 14-Hydroxy-cis-4,cis-7,cis-10,cis-16,cis-19-docosapentaenoic acid. 2. A method of producing 14-hydroxy-cis-4,cis-7,cis-10,cis-16,cis-19-docosapentaenoic acid, comprising incubating under suitable conditions cis-4,cis-7,cis-10,cis-13,cis-16,cis-19-docosahexaenoic acid with a hydratase of any of the following (a)-(c): (a) the protein consisting of the amino acid sequence shown in SEQ ID NO: 2, (b) a protein comprising an amino acid sequence wherein one to twenty amino acids in the amino acid sequence shown in SEQ ID NO: 2 are deleted and/or substituted and/or inserted and/or added, and having an enzyme activity that catalyzes a hydration reaction, and (c) a protein encoded by a nucleotide sequence that hybridizes to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1 under conditions comprising a wash in 0.1×SSC, 0.1% SDS at 68° C., and having an enzyme activity that catalyzes a hydration reaction. 3. A method of producing 14-hydroxy-cis-4,cis-7,cis-10,cis-16,cis-19-docosapentaenoic acid according to claim 2 , wherein the hydratase is the protein consisting of the amino acid sequence shown in SEQ ID NO: 2. 4. A method of producing 14-hydroxy-cis-4,cis-7,cis-10,cis-16,cis-19-docosapentaenoic acid according to claim 2 , wherein the hydratase is a protein comprising an amino acid sequence wherein one to twenty amino acids in the amino acid sequence shown in SEQ ID NO: 2 are deleted and/or substituted and/or inserted and/or added, and having an enzyme activity that catalyzes a hydration reaction. 5. A method of producing 14-hydroxy-cis-4,cis-7,cis-10,cis-16,cis-19-docosapentaenoic acid according to claim 2 , wherein the hydratase is a protein encoded by a nucleotide sequence that hybridizes to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1 under conditions comprising a wash in 0.1×SSC, 0.1% SDS at 68° C., and having an enzyme activity that catalyzes a hydration reaction.
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Classifications
- C12N9/0069Primary
acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13) · CPC title
Hydroxy-carboxylic acids · CPC title
Antihyperlipidemics · CPC title
acting on CH-OH groups as donors (1.1) · CPC title
- C12P7/6409Primary
Fatty acids · CPC title
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- What does patent US11046980B2 cover?
- The present invention provides production of hydroxylated fatty acid by a hydration reaction using a novel enzyme derived from Lactobacillus and using fatty acid as a substrate, and further, a production method of oxo fatty acid by an enzyme reaction or chemical oxidation reaction using the hydroxylated fatty acid as a substrate. In addition, a valuable novel rare fatty acid obtained by such pr…
- Who is the assignee on this patent?
- Univ Kyoto, Noster Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0069. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 29 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 4 related publications on this page (citations in our corpus or others sharing the same primary CPC).