Engineered imine reductases and methods for the reductive animation of ketone and amine compounds
US-9193957-B2 · Nov 24, 2015 · US
Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
US11046936B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11046936-B2 |
| Application number | US-201716319537-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 8, 2017 |
| Priority date | Aug 26, 2016 |
| Publication date | Jun 29, 2021 |
| Grant date | Jun 29, 2021 |
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- Title
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Abstract
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The present application provides engineered polypeptides having imine or oxime reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone amine substrate compounds to prepare secondary and tertiary amine product compounds.
First claim
Opening claim text (preview).
What is claimed is: 1. An engineered polypeptide comprising an amino acid sequence with at least 80% sequence identity to a reference sequence of SEQ ID NO: 14, and wherein said engineered polypeptide has imine reductase activity. 2. The engineered polypeptide of claim 1 , wherein said polypeptide further comprises at least one substitution selected from 57T/211L, 87S/175G, 103H/212C, 122E, 123G, 124A/178I, 124G, 132H/R, 134R, 171W, 173H/I, 175G/H, 176S, 178I/242Y, 178M, 211L/W, 212C/G/V, 213P/R/T/V, 214M, 215L/M, 217I/L, 234L, and 235K, wherein said positions are numbered with reference to SEQ ID NO:2. 3. The engineered polypeptide of claim 1 , wherein said polypeptide further comprises at least one substitution selected from A57T/I211L, P87S/M175G, R103H/E212C, L122E, T123G, I124A/V178I, I124G, E132H/R, T134R, Y171W, A173H/I, M175G/H, L176S, V178I/A242Y, V178M, I211L/W, E212C/G/V, A213P/R/T/V, V214M, T215L/M, W217I/L, D234L, and A235K, wherein said positions are numbered with reference to SEQ ID NO:2. 4. The engineered polypeptide of claim 1 , wherein said polypeptide comprises an amino acid sequence with at least 80% sequence identity to any even-numbered sequence set forth in SEQ ID NO:4 to SEQ ID NO:74. 5. A method of producing an engineered imine or oxime reductase polypeptide, comprising the steps of culturing a host cell, under conditions such that an engineered polynucleotide is expressed and said engineered polypeptide of claim 1 is produced. 6. The method of claim 5 , further comprising the step of recovering said engineered polypeptide.
Assignees
Inventors
Classifications
- C12N9/0036Primary
acting on NADH or NADPH (1.6) · CPC title
Oxidoreductases acting on NADH or NADPH (1.6) · CPC title
- C12N9/0028Primary
with NAD or NADP as acceptor (1.5.1) · CPC title
with NAD+ or NADP+ as acceptor (1.5.1) · CPC title
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- What does patent US11046936B2 cover?
- The present application provides engineered polypeptides having imine or oxime reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone amine substrate compounds to prepare secondary and tertiary amine product compounds.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0036. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 29 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).