Glucose dehydrogenase

Patent metadata

Field	Value
Publication number	US-11046935-B2
Application number	US-201615772661-A
Country	US
Kind code	B2
Filing date	Oct 26, 2016
Priority date	Nov 6, 2015
Publication date	Jun 29, 2021
Grant date	Jun 29, 2021

Abstract

Intended is to provide a highly practical novel FAD-GDH. A glucose dehydrogenase having the following properties is provided: (1) action: catalyzes the reaction of oxidizing hydroxyl groups of glucose to form glucono-δ-lactone in the presence of an electron acceptor; (2) substrate specificity: reactivity to D-xylose is 10% or less when the reactivity to D-glucose is 100%; (3) pH stability: stable at pH 5 to 8; (4) amino acid sequence: including the amino acid sequence set forth in SEQ ID NO: 1, or an amino acid sequence with an identity of 83% or more to the amino acid sequence.

First claim

The invention claimed is:

1. A recombinant DNA comprising a DNA selected from the group consisting of the following (A) and (B):
(A) a DNA comprising the nucleotide sequence set forth in SEQ ID NO: 2; and
(B) a DNA comprising a nucleotide sequence that has 90% or more sequence identity to the nucleotide sequence set forth in SEQ ID NO: 2, and encoding a protein having glucose dehydrogenase activity.

2. A microorganism comprising the recombinant DNA according to claim 1 .

3. A method for producing glucose dehydrogenase comprising the following steps (1) and (2), or the following steps (i) and (ii):
(1) culturing an Aspergillus iizukae NBRC 8869 strain in a culture solution, wherein the Aspergillus iizukae NBRC 8869 strain produces glucose dehydrogenase comprising the amino acid sequence of SEQ ID NO: 1; and
(2) collecting the glucose dehydrogenase from the culture solution and/or the Aspergillus iizukae NBRC 8869 fungal cells after culturing;
(i) culturing a microorganism to produce a protein having glucose dehydrogenase activity, wherein the microorganism is transformed with a plasmid comprising a polynucleotide with a nucleotide sequence encoding the protein having glucose dehydrogenase activity, wherein the nucleotide sequence encoding the protein having glucose dehydrogenase activity is selected from the group consisting of the following (a) to (d):
(a) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 1;
(b) a nucleotide sequence encoding an the amino acid sequence with 90% or more sequence identity to the amino acid sequence set forth in SEQ ID NO: 1 and having glucose dehydrogenase activity;
(c) the nucleotide sequence set forth in SEQ ID NO: 2; and
(d) a nucleotide sequence with 90% or more sequence identity to the nucleotide sequence set forth in SEQ ID NO: 2 and encoding a protein having glucose dehydrogenase activity; and

(ii) collecting the protein having glucose dehydrogenase activity produced in step (i).

Assignees

• Amano Enzyme Inc

Inventors

• Nishio Kyoichi
• Kojima Yuzo
• Yamaguchi Shotaro

Classifications

• G01N33/66

  involving blood sugars, e.g. galactose · CPC title

• C12Q1/32

  involving dehydrogenase · CPC title

• C12M1/34

  Measuring or testing with condition measuring or sensing means, e.g. colony counters · CPC title

• C12N5/10

  Cells modified by introduction of foreign genetic material · CPC title

• C12N15/09

  Recombinant DNA-technology · CPC title