Surgically-friendly tissue papers from organ-specific decellularized extracellular matrices

Patent metadata

Field	Value
Publication number	US-11045581-B2
Application number	US-201716461461-A
Country	US
Kind code	B2
Filing date	Nov 17, 2017
Priority date	Nov 18, 2016
Publication date	Jun 29, 2021
Grant date	Jun 29, 2021

Abstract

Provided herein are inks including decellularized extracellular matrix (dECM) particles and scaffolds made from the inks. Also provided are methods of making the scaffolds and applications for the scaffolds. In an embodiment, a porous scaffold comprises dECM particles and an elastomer, wherein the scaffold is planar having a thickness of about 100 μm or greater, the scaffold comprises irregularly shaped pores having a random orientation and distribution throughout the scaffold, and the scaffold is free of crosslinking between the molecular components of the scaffold.

First claim

What is claimed is:

1. A porous scaffold consisting essentially of:
decellularized extracellular matrix (dECM) particles and an elastomer;
wherein the scaffold is planar having a thickness of about 100 μm or greater;
wherein the scaffold comprises irregularly shaped pores having a random orientation and distribution throughout the scaffold;
wherein the scaffold is free of crosslinking between the molecular components of the scaffold and is not a gel;
wherein the scaffold is substantially free of liquid;
wherein the scaffold is mechanically robust enough to retain its structural integrity while undergoing cutting and multiple foldings in surgical applications, wherein retaining its structural integrity comprises not breaking, crumbling, flaking or tearing; and
wherein the porous scaffold is sized and configured for use with cells, a tissue, or an organ.

2. The scaffold of claim 1 , wherein the scaffold is a monolithic, continuous structure.

3. The scaffold of claim 1 , wherein the scaffold, in a dry state, exhibits a Young's modulus in the range of from about 1 MPa to about 30 MPa.

4. The scaffold of claim 1 , wherein the scaffold, in a dry state, exhibits an ultimate tensile strength of from about 0.1 MPa to about 1.5 MPa.

5. The scaffold of claim 1 , wherein the scaffold exhibits an absorbency in the range of from about 100% to about 500%.

6. The scaffold of claim 1 , the scaffold having from about 30% to about 80% by weight dECM particles, based on the total solids content of the scaffold, and from about 20% to about 70% by weight elastomer, based on the total solids content of the scaffold.

7. The scaffold of claim 1 , wherein the scaffold is capable of supporting a population of human mesenchymal stem cells, hepatocytes or ovarian follicles, the population which retains its viability over a time period of at least 28 days when the scaffold is cultured in vitro.

8. The scaffold of claim 1 , wherein the scaffold is capable of supporting ovarian tissue which retains its viability over a time period of at least 8 weeks and is capable of supporting the development of one or more vasa-positive oocytes from the ovarian tissue, when the scaffold is cultured in vitro.

9. The scaffold of claim 8 , wherein the dECM particles are derived from an ovary.

10. The scaffold of claim 1 , wherein the scaffold is capable of supporting muscle tissue which retains its viability over a time period of at least 6 weeks when the scaffold is implanted in a mammalian subject in vivo.

11. The scaffold of claim 10 , wherein the dECM particles are derived from muscle, liver, heart, kidney, uterus, skin, or collagen.

12. The scaffold of claim 1 , the scaffold having an external topography that is complementary to a pattern mold.

Assignees

• Univ Northwestern

Inventors

• Jakus Adam E
• Shah Ramille N

Classifications

• B33Y70/00

  Materials specially adapted for additive manufacturing · CPC title

• A61L27/3895

  using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions · CPC title

• A61L27/48

  with macromolecular fillers · CPC title

• A61L27/56

  Porous materials, {e.g. foams or sponges} · CPC title

• A61L2430/22

  for reconstruction of hollow organs, e.g. bladder, esophagus, urether, uterus · CPC title