Compositions comprising recombinant Bacillus cells and an insecticide

Patent metadata

Field	Value
Publication number	US-11044916-B2
Application number	US-201515511864-A
Country	US
Kind code	B2
Filing date	Sep 17, 2015
Priority date	Sep 17, 2014
Publication date	Jun 29, 2021
Grant date	Jun 29, 2021

Abstract

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.

First claim

What is claimed is:

1. A composition comprising:
(a) recombinant exosporium-producing Bacillus cells of a Bacillus cereus family member that express a fusion protein comprising:
(i) an aminocyclopropane-1-carboxylic acid deaminase; and
(ii) a targeting sequence, or an exosporium protein, wherein the targeting sequence or the exosporium protein comprises:
an amino acid sequence having at least 43% sequence identity with amino acids 20-35 of SEQ ID NO: 1, wherein the sequence identity with amino acids 25-35 of SEQ ID NO: 1 is at least 54%;
a targeting sequence comprising amino acids 1-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-31 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-33 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-31 of SEQ ID NO: 1;
a targeting sequence comprising SEQ ID NO: 1; or
an exosporium protein comprising an amino acid sequence having at least 85% sequence identity with SEQ ID NO: 2 and
(b) at least one insecticide selected from the group consisting of abamectin, carbofuran, cyazypyr, cycloaprid, spinosad, sulfoxaflor, and tefluthrin, in a synergistically effective amount.

2. The composition of claim 1 , wherein the recombinant Bacillus cells are from Bacillus thuringiensis BT013A.

3. The composition according to claim 1 , wherein the at least one insecticide is spinosad.

4. The composition according to claim 1 , wherein the at least one insecticide is abamectin.

5. The composition according to claim 1 , wherein the at least one insecticide is carbofuran.

6. A seed treated with the composition according to claim 1 .

7. A method of treating a plant, a plant part, or the locus surrounding the plant to enhance plant growth and/or promote plant health comprising the step of simultaneously or sequentially applying:
a) recombinant exosporium-producing Bacillus cells of a Bacillus cereus family member that express a fusion protein comprising:
(i) an aminocyclopropane-1-carboxylic acid deaminase; and
(ii) a targeting sequence, or an exosporium protein, wherein the targeting sequence or the exosporium protein comprises:
an amino acid sequence having at least 43% sequence identity with amino acids 20-35 of SEQ ID NO: 1, wherein the sequence identity with amino acids 25-35 of SEQ ID NO: 1 is at least 54%;
a targeting sequence comprising amino acids 1-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-31 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-33 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-31 of SEQ ID NO: 1;
a targeting sequence comprising SEQ ID NO: 1; or
an exosporium protein comprising an amino acid sequence having at least 85% sequence identity with SEQ ID NO: 2 and
(b) at least one insecticide selected from the group consisting of abamectin, carbofuran, cyazypyr, cycloaprid, spinosad, sulfoxaflor, and tefluthrin, in a synergistically effective amount.

Assignees

• Bayer Cropscience Lp
• Spogen Biotech Inc

Inventors

• Curtis Damian
• Thompson Brian

Classifications

• A61K39/07 Primary

  Bacillus · CPC title

• C12Y301/01004

  Phospholipase A2 (3.1.1.4) · CPC title

• C12Y301/01 Primary

  Carboxylic ester hydrolases (3.1.1) · CPC title

• C12Y302/01004

  Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title

• A61K35/742

  Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes · CPC title