Methods of creating drought tolerant corn plants using markers linked to cold shock domain-containing proteins and compositions thereof

Patent metadata

Field	Value
Publication number	US-11032986-B2
Application number	US-201916445041-A
Country	US
Kind code	B2
Filing date	Jun 18, 2019
Priority date	Nov 7, 2012
Publication date	Jun 15, 2021
Grant date	Jun 15, 2021

Abstract

The present invention is in the field of plant breeding and drought tolerance. More specifically, the invention includes a method for breeding corn plants containing one or more markers that are associated with tolerance to low water conditions. Markers provided herein include markers genetically linked to proteins comprising cold shock domains, such as the CSD1 and CSD2 proteins. The invention further includes germplasm and the use of germplasm containing at least one marker associated with drought tolerance for introgression into elite germplasm in a breeding program, thus producing drought resistant germplasm. Other embodiments of this invention include transgenic drought tolerant corn plants and corn plant cells, and methods of detecting drought tolerance genes and chromosome regions associated with drought tolerance in a plant genome.

First claim

What is claimed is:

1. A method of creating a population of corn plants with enhanced drought tolerance, said method comprising:
a. providing a first population of corn plants;
b. detecting the presence of an enhanced drought tolerance allele, wherein said allele is within 5 cM of a nucleotide corresponding to position 3776 of SEQ ID NO. 2 in the first population, and wherein said nucleotide at said position is associated with an enhanced drought tolerance phenotype;
c. selecting one or more corn plants based on the presence of the enhanced drought tolerance allele from the first population of corn plants; and
d. producing a population of offspring from the one or more selected corn plants wherein the offspring population exhibits enhanced drought tolerance as compared to the first population.

2. The method of claim 1 , wherein said detecting the presence of the enhanced drought tolerance allele comprises detecting a genetic marker within SEQ ID NO. 2.

3. A method of increasing the frequency of a drought tolerance phenotype in a population of corn plants, said method comprising:
a. providing a first population of corn plants;
b. detecting the presence of an enhanced drought tolerance allele, wherein said allele is within 5 cM of a nucleotide corresponding to position 3776 of SEQ ID NO. 2 in the first population, and wherein said nucleotide at said position is associated with an enhanced drought tolerance phenotype;
c. selecting one or more corn plants based on the presence of the enhanced drought tolerance allele from the first population of corn plants; and
d. producing an offspring population from the one or more selected corn plants such that the drought tolerance phenotype occurs more frequently in the offspring population as compared to the first population.

4. The method of claim 3 , wherein the detecting the presence of the enhanced drought tolerance allele comprises detecting a genetic marker within SEQ ID NO. 2.

5. The method of claim 1 , wherein said providing a first population of corn plants comprises crossing a corn plant comprising said enhanced drought tolerance allele with a second corn plant of a different genotype lacking said allele.

6. The method of claim 1 , wherein the population of corn plants comprises F 2 -F 6 progeny plants.

7. The method of claim 1 , wherein said providing a first population of corn plants comprises backcrossing.

8. The method of claim 7 , wherein backcrossing comprises marker-assisted selection in at least two generations.

9. The method of claim 8 , wherein backcrossing comprises marker-assisted selection in all generations.

10. The method of claim 1 , further comprising assaying the selected one or more corn plants comprising said allele or a progeny thereof comprising the allele for said enhanced drought tolerance.

11. The method of claim 3 , wherein said providing a first population of corn plants comprises crossing a corn plant comprising at least a first enhanced drought tolerance allele with a second corn plant of a different genotype lacking said allele to produce progeny plants.

12. The method of claim 3 , wherein the population of corn plants comprises F 2 -F 6 progeny plants.

13. The method of claim 3 , wherein said providing a first population of corn plants comprises backcrossing.

14. The method of claim 13 , wherein backcrossing comprises marker-assisted selection in at least two generations.

15. The method of claim 14 , wherein backcrossing comprises marker-assisted selection in all generations.

16. The method of claim 3 , further comprising assaying the selected one or more corn plants comprising said allele or a progeny thereof comprising the allele for said enhanced drought tolerance.

Assignees

• Monsanto Technology Llc

Inventors

• Chomet Paul S
• Houmard Nancy M
• Jia Hongwu
• Kerns Michael R
• Leibman Mark E

Classifications

• A01H5/10 Primary

  Seeds · CPC title

• A01H6/4684

  Zea mays [maize] · CPC title

• A01H1/04 Primary

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title

• C12N15/8273

  for drought, cold, salt resistance · CPC title