Data directed DESI-MS imaging

Patent metadata

Field	Value
Publication number	US-11031230-B2
Application number	US-201716306809-A
Country	US
Kind code	B2
Filing date	Jun 5, 2017
Priority date	Jun 3, 2016
Publication date	Jun 8, 2021
Grant date	Jun 8, 2021

Abstract

A method of analysing a sample is disclosed that comprises surveying a sample in a first mode of operation by directing a spray of charged droplets onto the sample, determining one or more regions of interest in the sample, and analysing the one or more regions of interest in a second different mode of operation by directing a spray of charged droplets onto the sample. The spot size of the spray of charged droplets at a point of impact with the sample may be varied.

First claim

The invention claimed is:

1. A method of analysing a sample comprising:
(i) surveying a sample in a first mode of operation by directing a spray of charged droplets onto said sample when said spray has a first cross-sectional area or first pixel size at a point of impact with said sample and scanning said spray of charged droplets across said sample at a first speed;
(ii) determining one or more regions of interest in said sample; and
(iii) analysing said one or more regions of interest in a second different mode of operation by directing a spray of charged droplets onto said sample when said spray has a second different cross-sectional area or second different pixel size at a point of impact with said sample and scanning said spray of charged droplets across said one or more regions of interest at a second different speed.

2. A method as claimed in claim 1 , wherein:
the step of (i) surveying said sample in said first mode of operation comprises surveying said sample by directing said spray of charged droplets onto a plurality of first target regions of said sample, wherein said spray of charged droplets is directed onto each of said plurality of first target regions for a first dwell time; and
the step of (iii) analysing said one or more regions of interest in said second different mode of operation comprises analysing said one or more regions of interest by directing said spray of charged droplets onto a plurality of second target regions of said one or more regions of interest, wherein said spray of charged droplets is directed onto each of said plurality of second target regions for a second different dwell time.

3. A method as claimed in claim 1 , wherein:
the step of (i) surveying said sample in said first mode of operation comprises surveying said sample in said first mode of operation during a first time period; and
the step of (iii) analysing said one or more regions of interest in said second different mode of operation comprises analysing said one or more regions of interest in said second mode of operation during a second time period.

4. A method as claimed in claim 1 , wherein:
the step of (i) surveying said sample in said first mode of operation comprises directing said spray of charged droplets onto one or more first regions of said sample; and
the step of (iii) analysing said one or more regions of interest in said second different mode of operation comprises directing said spray of charged droplets onto said one or more regions of interest;
wherein at least some of said one or more regions of interest are the same as or overlap with at least some of said one or more first regions.

5. A method as claimed in claim 1 , wherein:
the step of (i) surveying said sample in said first mode of operation comprises directing said spray of charged droplets onto said sample, wherein said charged droplets have a first polarity; and
the step of (iii) analysing said one or more regions of interest in said second different mode of operation comprises directing said spray of charged droplets onto said sample, wherein said charged droplets have a second different polarity.

6. A method as claimed in claim 1 , wherein:
the step of (i) surveying said sample in said first mode of operation comprises directing said spray of charged droplets onto said sample, wherein said charged droplets comprise a first solvent or solvent composition; and
the step of (iii) analysing said one or more regions of interest in said second different mode of operation comprises directing said spray of charged droplets onto said sample, wherein said charged droplets comprise a second different solvent or solvent composition.

7. A method as claimed in claim 1 , wherein said first and/or second mode of operation comprises: (i) a mass spectrometry (“MS”) mode of operation; (ii) a tandem mass spectrometry (“MS/MS”) mode of operation; (iii) a mode of operation in which parent or precursor ions are alternatively fragmented or reacted to produce fragment or product ions, and not fragmented or reacted or fragmented or reacted to a lesser degree; (iv) a Multiple Reaction Monitoring (“MRM”) mode of operation; (v) a Data Dependent Analysis (“DDA”) mode of operation; (vi) a Data Independent Analysis (“DIA”) mode of operation; (vii) a Quantification mode of operation; or (viii) an Ion Mobility Spectrometry (“IMS”) mode of operation.

8. A method as claimed in claim 1 , wherein said first and/or second mode of operation comprises: (i) a Collisional Induced Dissociation (“CID”) mode of operation; (ii) a Surface Induced Dissociation (“SID”) mode of operation; (iii) an Electron Transfer Dissociation (“ETD”) mode of operation; (iv) an Electron Capture Dissociation (“ECD”) mode of operation; (v) an Electron Collision or Impact Dissociation mode of operation; (vi) a Photo Induced Dissociation (“PID”) mode of operation; (vii) a Laser Induced Dissociation mode of operation; (viii) an infrared radiation induced dissociation mode of operation; (ix) an ultraviolet radiation induced dissociation mode of operation; (x) a nozzle-skimmer interface fragmentation mode of operation; (xi) an in-source fragmentation mode of operation; (xii) an in-source Collision Induced Dissociation mode of operation; (xiii) a thermal fragmentation mode of operation; (xiv) an electric field induced fragmentation mode of operation; (xv) a magnetic field induced fragmentation mode of operation; (xvi) an enzyme digestion or enzyme degradation fragmentation mode of operation; (xvii) an ion-ion reaction fragmentation mode of operation; (xviii) an ion-molecule reaction fragmentation mode of operation; (xix) an ion-atom reaction fragmentation mode of operation; (xx) an ion-metastable ion reaction fragmentation mode of operation; (xxi) an ion-metastable molecule reaction fragmentation mode of operation; (xxii) an ion-metastable atom reaction fragmentation mode of operation; (xxiii) an ion-ion reaction mode of operation wherein ions react to form adduct or product ions; (xxiv) an ion-molecule reaction mode of operation wherein ions react to form adduct or product ions; (xxv) an ion-atom reaction mode of operation wherein ions react to form adduct or product ions; (xxvi) an ion-metastable ion reaction mode of operation wherein ions react to form adduct or product ions; (xxvii) an ion-metastable molecule reaction mode of operation wherein ions react to form adduct or product ions; (xxviii) an ion-metastable atom reaction mode of operation wherein ions react to form adduct or product ions; or (xxix) an Electron Ionisation Dissociation (“EID”) mode of operation.

9. A method as claimed in claim 1 , further comprising selecting and/or optimising said second mode of operation based on information acquired during said first mode of operation.

10. A method as claimed in claim 1 , wherein the step of (i) surveying said sample in said first mode of operation comprises surveying said sample and one or more regions surrounding said sample by directing said spray of charged droplets onto said sample and onto said one or more regions surrounding said sample.

11. A method as claimed in claim 1 , wherein said sample is mounted on a substrate or slide, and the step of (i) surveying said sample in said first mode of operation comprises: surveying most or all of the area of said substrate or slide including said sample.

12. A method as claimed in claim 1 , wherein the step of (ii) determining one or more regions of interest in said sample comprises determining one or more boundaries of said sample.

13. A method as claimed in claim 1 , wherein the step of (iii) analysing said one or more regions of interest in said second different mode of operation comprises: ana

Assignees

• Micromass Ltd

Inventors

• Jones Emrys
• Chapman Richard
• Pringle Steven Derek

Classifications

• H01J49/0004 Primary

  Imaging particle spectrometry · CPC title

• H01J49/142

  using a solid target which is not previously vapourised · CPC title

• H01J49/28

  Static spectrometers · CPC title

• H01J49/165 Primary

  Electrospray ionisation · CPC title