Insecticidal polypeptides having improved activity spectrum and uses thereof
US-2017226164-A1 · Aug 10, 2017 · US
Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof
US11028407B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11028407-B2 |
| Application number | US-201716092341-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 19, 2017 |
| Priority date | Apr 19, 2016 |
| Publication date | Jun 8, 2021 |
| Grant date | Jun 8, 2021 |
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Abstract
Official abstract text for this publication.
The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
First claim
Opening claim text (preview).
That which is claimed: 1. A DNA construct comprising a nucleic acid molecule encoding a variant Cry1B polypeptide having at least 95% sequence identity to SEQ ID NO: 29 and having insecticidal activity; and a nucleic acid molecule encoding a PtIP-83 polypeptide having at least 95% sequence identity to SEQ ID NO: 62, 64, or 66 and having insecticidal activity. 2. The DNA construct of claim 1 , wherein the nucleic acid molecules encoding the variant Cry1B polypeptide and the PtIP-83 polypeptide are each operably linked to a heterologous regulatory element. 3. A transgenic plant comprising a molecular stack of a nucleic acid molecule encoding a variant Cry1B polypeptide having at least 95% sequence identity to SEQ ID NO: 29 and having insecticidal activity; and a nucleic acid molecule encoding a PtIP-83 polypeptide having at least 95% sequence identity to SEQ ID NO: 62, 64, or 66 and having insecticidal activity. 4. The transgenic plant of claim 3 , wherein the nucleic acid molecules encoding the variant Cry1B polypeptide and the PtIP-83 polypeptide are each operably linked to a heterologous regulatory element. 5. A transgenic plant comprising a breeding stack of a nucleic acid molecule encoding a variant Cry1B polypeptide having at least 95% sequence identity to SEQ ID NO: 29 and having insecticidal activity; and a nucleic acid molecule encoding a PtIP-83 polypeptide having at least 95% sequence identity to SEQ ID NO: 62, 64, or 66 and having insecticidal activity. 6. The transgenic plant of claim 5 , wherein the nucleic acid molecule encoding the variant Cry1B polypeptide and the PtIP-83 polypeptide are each operably linked to a heterologous regulatory element. 7. A transgenic plant or progeny thereof comprising the DNA construct of claim 1 , wherein said transgenic plant is corn or soy. 8. The transgenic plant or progeny thereof comprising the molecular stack of claim 3 , wherein said transgenic plant is corn or soy. 9. The transgenic plant or progeny thereof comprising the breeding stack of claim 5 , wherein said transgenic plant is corn or soy. 10. A composition comprising a nucleic acid molecule encoding a variant Cry1B polypeptide having at least 95% sequence identity to SEQ ID NO: 29 and having insecticidal activity and a nucleic acid molecule encoding a PtIP-83 polypeptide having at least 95% sequence identity to SEQ ID NO: 62, 64, or 66 and having insecticidal activity.
Assignees
Inventors
Classifications
Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title
Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title
- C12N15/8286Primary
for insect resistance · CPC title
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Frequently asked questions
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- What does patent US11028407B2 cover?
- The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and …
- Who is the assignee on this patent?
- Pioneer Hi Bred Int, Du Pont
- What technology area does this patent fall under?
- Primary CPC classification C12N15/8286. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 08 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).