Multisignal labeling reagents and processes and uses therefor

Patent metadata

Field	Value
Publication number	US-11028121-B2
Application number	US-202016830483-A
Country	US
Kind code	B2
Filing date	Mar 26, 2020
Priority date	Apr 3, 2003
Publication date	Jun 8, 2021
Grant date	Jun 8, 2021

Abstract

Provided are compounds comprising two DNA supramolecular binding molecules covalently joined by a linker group. Also provided are multisignal labeling reagents comprising (i) an oligomer of nucleotides or nucleotide analogs; (ii) a DNA supramolecular binding molecule noncovalently bound to the oligomer; and (iii) a first reactive group or a first partner of a first binding pair covalently bound to the oligomer. Additionally provided are methods of producing multisignal labeling reagents.

First claim

What is claimed is:

1. A multi-signal labeling reagent, comprising:
a plurality of first nucleic acid strands covalently bound to the first member of a binding pair and not to the second member of the binding pair; and
a plurality of second nucleic acid strands covalently bound to a second member of the binding pair and not to the first member of the binding pair,
wherein at least some of the nucleic acid monomers of one or both of the first nucleic acid strands and the second nucleic acid strands comprise a covalently linked signal moiety, and
wherein the binding pair is not a nucleic acid binding pair.

2. The multi-signal labeling reagent of claim 1 , wherein the signal moiety comprises a fluorescent moiety, a phosphorescent moiety or a chemiluminescent moiety.

3. The multi-signal labeling reagent of claim 1 , wherein each of the first nucleic acid strand and the second nucleic acid strand comprise nucleic acid monomers comprising a covalently linked signal moiety.

4. The multi-signal labeling reagent of claim 3 , wherein the signal moiety comprises a fluorescent moiety, a phosphorescent moiety or a chemiluminescent moiety.

5. The multi-signal labeling reagent of claim 1 , wherein the binding pair is selected from biotin and streptavidin or biotin and avidin.

6. The multi-signal labeling reagent of claim 2 , wherein the binding pair is selected from biotin and streptavidin or biotin and avidin.

7. The multi-signal labeling reagent of claim 3 , wherein the binding pair is selected from biotin and streptavidin or biotin and avidin.

8. The multi-signal labeling reagent of claim 4 , wherein the binding pair is selected from biotin and streptavidin or biotin and avidin.

Assignees

• Enzo Life Science Inc
• Enzo Life Sciences Inc

Inventors

• Coleman Jack
• Rabbani Elazar
• Stavrianopoulos Jannis
• Pande Praveen

Classifications

• C07D405/14

  containing three or more hetero rings · CPC title

• C09B11/08

  Phthaleins; {Phenolphthaleins; Fluorescein} · CPC title

• C09B11/00

  Diaryl- or thriarylmethane dyes · CPC title

• C07D219/08

  Nitrogen atoms · CPC title

• C09B11/24

  Phthaleins containing amino groups {; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes} · CPC title