Method of producing thin enzyme-based sensing layers on planar sensors

Patent metadata

Field	Value
Publication number	US-11026610-B2
Application number	US-201816144079-A
Country	US
Kind code	B2
Filing date	Sep 27, 2018
Priority date	Sep 28, 2017
Publication date	Jun 8, 2021
Grant date	Jun 8, 2021

Abstract

A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

First claim

What is claimed is:

1. A method, comprising:
depositing a masking layer onto a device comprising a plurality of electrodes, wherein the masking layer covers the plurality of electrodes;
depositing an adhesion promoter onto the device and onto the masking layer, wherein deposition of the adhesion promoter onto the plurality of electrodes is avoided due to the masking layer;
removing the masking layer from the device coated with the adhesion promoter;
after removing the masking layer, spin coating a signal transduction enzyme onto the device, the signal transduction enzyme being configured to detect an active compound capable of generating an electric current; and
crosslinking the signal transduction enzyme by depositing a crosslinker onto the spin coated signal transduction enzyme to produce a cross-linked enzyme film having a thickness of less than 1 micrometer, and
wherein the signal transduction enzyme is configured so that a current measured by the plurality of electrodes settles within about 5% of its stable value within 1 second.

2. The method of claim 1 , further comprising, prior to spin coating the signal transduction enzyme onto the device: mixing the signal transduction enzyme in a solvent with at least one non-reactive component.

3. The method of claim 2 , wherein the at least one non-reactive component comprises a filler, a buffer salt to regulate pH, or both the filler and the buffer salt.

4. The method of claim 3 , wherein the crosslinking the signal transduction enzyme is by:
placing the device, coated with the signal transduction enzyme, and the crosslinker in a vacuum chamber; and
reducing pressure within the vacuum chamber, thereby causing the crosslinker to vaporize and deposit onto the signal transduction enzyme.

5. The method of claim 2 , wherein the solvent is water.

6. The method of claim 5 , wherein the signal transduction enzyme is an oxidase enzyme.

7. The method of claim 6 , wherein the signal transduction enzyme is selected from the group consisting of: malate oxidase, hexose oxidase, aryl-alcohol oxidase, L-gulonolactone oxidase, pyranose oxidase, L-sorbose oxidase, pyridoxine 4-oxidase, (S)-2-hydroxy-acid oxidase, ecdysone oxidase, secondary-alcohol oxidase, 4 hydroxymandelate oxidase, long-chain-alcohol oxidase, thiamine oxidase, hydroxyphytanate oxidase, N-acylhexosamine oxidase, polyvinyl-alcohol oxidase, D-Arabinono-1,4-lactone oxidase, vanillyl-alcohol oxidase, D-mannitol oxidase, alditol oxidase, choline dehydrogenase, gluconate 2-dehydrogenase glucooligosaccharide oxidase, alcohol dehydrogenase, cellobiose dehydrogenase, aldehyde oxidase, glyoxylate oxidase, indole-3-acetaldehyde oxidase, aryl-aldehyde oxidase, retinal oxidase, abscisic-aldehyde oxidase, aldehyde ferredoxin oxidoreductase, indolepyruvate ferredoxin oxidoreductase, aldehyde dehydrogenase, dihydroorotate oxidase, acyl-CoA oxidase, dihydrouracil oxidase, tetrahydroberberine oxidase, tryptophan alpha,beta-oxidase, L-galactonolactone oxidase, acyl-CoA dehydrogenase, Isoquinoline-1-oxidoreductase, quinaldate 4-oxidoreductase, D-aspartate oxidase, L-amino-acid oxidase, monoamine oxidase, pyridoxal 5′-phosphate synthase, D-glutamate oxidase, ethanolamine oxidase, putrescine oxidase, cyclohexylamine oxidase, protein-lysine 6-oxidase, L-lysine 6-oxidase, primary-amine oxidase, 7 chloro-L-tryptophan oxidase, N-methyl-L-amino-acid oxidase, non-specific polyamine oxidase, N8-acetylspermidine oxidase, N6-methyl-lysine oxidase, polyamine oxidase, N1-acetylpolyamine oxidase, spermine oxidase, pipecolate oxidase, dimethylglycine oxidase, polyamine oxidase, Dihydrobenzophenanthridine oxidase, urate oxidase, 3-aci-nitropropanoate oxidase, sulfite oxidase, methanethiol oxidase, prenylcysteine oxidase, L-ascorbate oxidase, 3 hydroxyanthranilate oxidase, rifamycin-B oxidase, superoxide dismutase, reticuline oxidase, lactate oxidase, D-amino acid oxidase, (S)-6-hydroxynicotine oxidase, (R)-6-hydroxynicotine oxidase, alcohol oxidase, pyruvate oxidase, glucose oxidase, L-glutamate oxidase, acyl coenzyme A oxidase, choline oxidase, glutathione sulfhydryl oxidase, glycerolphosphate oxidase, sarcosine oxidase, xanthine oxidase, oxalate oxidase, cholesterol oxidase, gamma-glutamyl-putrescine oxidase, GABA oxidase, histamine oxidase, nucleoside oxidase, L-lysine oxidase, L-aspartate oxidase, glycine oxidase, and galactose oxidase.

8. The method of claim 6 , wherein the signal transduction enzyme is selected from the group consisting of: Lactate oxidase, D-amino acid oxidase, (S)-6-Hydroxynicotine oxidase, (R)-6-Hydroxynicotine oxidase, Alcohol oxidase, Pyruvate oxidase, Glucose oxidase, Glutamate oxidase, Acyl coenzyme A oxidase, Choline oxidase, Glutathione Sulfhydryl oxidase, Glycerolphosphate oxidase, Sarcosine oxidase, Xanthine oxidase, Oxalate oxidase, Cholesterol oxidase, Gamma-glutamyl-putrescine oxidase, GABA oxidase, Histamine oxidase, Nucleoside oxidase, L-Lysine oxidase, L-Aspartate oxidase, Glycine oxidase, Urate oxidase, and Galactose oxidase.

9. The method of claim 1 , wherein the crosslinker is glutaraldehyde.

10. The method of claim 1 , wherein the adhesion promoter is an inorganic-organic amine coupler.

11. The method of claim 1 , wherein the device comprises silicon.

12. The method of claim 1 , where the adhesion promoter is an aminosilane.

13. The method of claim 1 , where the adhesion promoter is 3-aminopropyltriethoxysilane (APTES), or 3-aminopropyltrimethoxysilane (APTMS).

14. The method of claim 1 , wherein the plurality of electrodes comprises a working electrode, a reference electrode, and a counter electrode.

Assignees

• California Inst Of Techn

Inventors

• Chen Samson
• Scherer Axel
• Adalian Dvin
• Petillo Peter
• Jilani Muhammad Musab
• Madero Xiomara L
• Kishore Kumar Deepan

Classifications

• G01N27/3272

  Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52) · CPC title

• A61B5/1459

  invasive, e.g. introduced into the body by a catheter · CPC title

• C12N11/14

  Enzymes or microbial cells immobilised on or in an inorganic carrier · CPC title

• A61B5/14532

  for measuring glucose, e.g. by tissue impedance measurement · CPC title

• C12Y101/03007

  Aryl-alcohol oxidase (1.1.3.7) · CPC title