High-throughput screening system based on multi-manipulators

Patent metadata

Field	Value
Publication number	US-11022622-B2
Application number	US-201715708955-A
Country	US
Kind code	B2
Filing date	Sep 19, 2017
Priority date	Jun 30, 2017
Publication date	Jun 1, 2021
Grant date	Jun 1, 2021

Abstract

The invention provides a high-throughput screening system based on multi-manipulators, and belongs to the field of biotechnology and detection equipment. A high-throughput screening system based on multi-manipulators, comprises of the first manipulator, sampler, pipette, plate washer, microplate reader, the second manipulator, centrifuge, deep-well plate library, waste shallow-well plate barrel, shallow-well plate library, waste needle plate barrel, needle library, waste deep-well plate barrel, collection box. The present invention is a combination of microbiology and mechanics. The aim of the invention is to realize the automation and intelligentization of the high throughput screening experiment, effectively improve the experimental accuracy, reliability and efficiency. It contributes to the development of high throughput screening technology for microorganisms and drugs.

First claim

What is claimed is:

1. A high-throughput screening system, comprising:
a first manipulator and a second manipulator each installed on a horizontal table and each configured to handle at least one 96-well microplate,
a sampler,
a pipette,
a 96-well microplate washer,
a 96-well microplate reader,
a centrifuge,
a first 96-well microplate library,
a waste second 96-well microplate barrel configured to hold a second 96-well microplate,
a second 96-well microplate library,
a waste needle microplate barrel configured to collect used sampling needles,
a needle library configured with space for storing sampling needles fitting the sampler and the pipette,
a waste first 96-well microplate barrel configured to hold a first 96-well microplate, and
a collection box configured to collect residual liquid from the first 96-well microplate;
wherein the collection box, the sampler, the pipette, the 96-well microplate washer, the 96-well microplate reader, the first 96-well microplate library, the waste second 96-well microplate barrel, the waste needle microplate barrel, the second 96-well microplate library, the needle library, and the waste first microplate barrel are placed on the horizontal table surrounding the first and second manipulators;
wherein the first 96-well microplate and the second 96-well microplate are each comprised of 96 uniform wells, and wherein each of the wells of the first 96-well microplate holds more liquid than each of the wells of the second 96-well microplate.

2. The high-throughput screening system according to claim 1 , wherein the sampler and pipette are capable of liquid extraction and pipetting and are configured to draw samples from the first 96-well microplate and to transfer the samples to the second 96-well microplate.

3. The high-throughput screening system according to claim 1 , wherein the microplate reader comprises a system integrated automatic digital microscope or microplate detector.

4. The high-throughput screening system according to claim 1 , wherein the first manipulator and the second manipulator cooperate with each other to complete automatic loading, unloading and transferring of the first 96-well microplate and the second 96-well microplate.

5. The high-throughput screening system according to claim 1 , wherein the first 96-well microplate library comprises a base plate with a first groove and a second groove that are configured for holding 96-well microplates; wherein the first groove and the second groove are adjacent and are configured to allow the manipulators to catch the 96-well microplates in the grooves.

6. The high-throughput screening system according to claim 1 , wherein the second 96-well microplate library comprises a base plate with grooves configured for holding 96-well microplates.

7. The high-throughput screening system according to claim 1 , wherein the manipulators comprise a gripper configured to hold both sides of a workpiece.

Assignees

• Univ Jiangnan

Inventors

• Zhou Jingwen
• Chen Jian
• Ning Meng
• Du Guocheng
• Zeng Weizhu
• Cao Xiaomei
• Zhang Qiuju
• Fang Jun

Classifications

• G01N1/31

  Apparatus therefor · CPC title

• G01N2035/0418

  Plate elements with several rows of samples · CPC title

• B01L9/50

  Clamping means, e.g. tongs · CPC title

• G01N35/10

  Devices for transferring samples {or any liquids} to, in, or from, the analysis apparatus, e.g. suction devices, injection devices {(G01N35/0099 takes precedence)} · CPC title

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title