Microfluidic tissue biopsy and immune response drug evaluation devices and systems

Patent metadata

Field	Value
Publication number	US-11022603-B2
Application number	US-201816116656-A
Country	US
Kind code	B2
Filing date	Aug 29, 2018
Priority date	Aug 30, 2017
Publication date	Jun 1, 2021
Grant date	Jun 1, 2021

Abstract

This disclosure describes microfluidic tissue biopsy and immune response drug evaluation devices and systems. A microfluidic device can include an inlet channel having a first end configured to receive a fluid sample optionally containing a tissue sample. The microfluidic device can also include a tissue trapping region at the second end of the inlet channel downstream from the first end. The tissue trapping region can include one or more tissue traps configured to catch a tissue sample flowing through the inlet channel such that the fluid sample contacts the tissue trap. The microfluidic device can also include one or more channels providing an outlet.

First claim

The invention claimed is:

1. A microfluidic device comprising:
a substrate defining:
an inlet channel having a first end configured to receive a fluid sample optionally containing a tissue sample;
a tissue trapping region at a second end of the inlet channel downstream from the first end, the tissue trapping region including one or more tissue traps configured to catch the tissue sample flowing through the inlet channel such that the fluid sample contacts the one or more tissue traps;
a first branch channel coupled to the second end of the inlet channel at a first junction and configured to direct a first portion of the fluid sample in a first direction;
a second branch channel coupled to the second end of the inlet channel at the first junction and configured to direct a second portion of the fluid sample in a second direction, different from the first direction, wherein at least one of the one or more tissue traps is positioned at the first junction;
a first suction channel coupling at least one of the one or more tissue traps to the first branch channel at a third junction downstream from the second end of the inlet channel; and
a second suction channel coupling at least one of the one or more tissue traps to the second branch channel at a fourth junction downstream from the second end of the inlet channel.

2. The microfluidic device of claim 1 , wherein at least one of the one or more tissue traps comprises an arrangement of one or more walls.

3. The microfluidic device of claim 1 , wherein at least one of the one or more tissue traps includes a bottom surface positioned at a lower depth than a bottom surface of the inlet channel.

4. The microfluidic device of claim 1 , wherein the first branch channel and the second branch channel converge at a second junction downstream from the one or more tissue traps.

5. The microfluidic device of claim 1 , wherein a diameter of at least one of the one or more tissue traps is about twice that of the inlet channel.

6. The microfluidic device of claim 1 , further comprising an inlet port coupled to the first end of the inlet channel and configured to deliver the fluid sample to the inlet channel.

7. The microfluidic device of claim 6 , wherein the inlet port comprises a first threaded connector configured for attachment to a fluid line.

8. The microfluidic device of claim 6 , further comprising a bubble trapping structure coupled to the inlet channel downstream from the inlet port, the bubble trapping structure configured to facilitate evacuation of air bubbles from the fluid sample.

9. The microfluidic device of claim 8 , wherein a surface of the bubble trapping structure has a shape defined by a parabolic function.

10. The microfluidic device of claim 8 , wherein the bubble trapping structure further comprises a second threaded connector configured for attachment to an air release line.

11. The microfluidic device of claim 1 , further comprising an outlet port coupled to at least one of the first branch channel or the second branch channel providing an outlet and configured to remove the fluid sample from the microfluidic device.

12. The microfluidic device of claim 1 , wherein the substrate is formed from a biocompatible material.

13. The microfluidic device of claim 1 , wherein the substrate is formed from an optically transparent material, the microfluidic device further comprising:
an optical interface providing optical access to the tissue sample positioned within the tissue trapping region.

14. The microfluidic device of claim 1 , wherein the one or more tissue traps are configured to entrain the tissue sample in place within the one or more tissue traps.

15. A microfluidic device, comprising:
a substrate defining:
an inlet channel having a first end configured to receive a fluid sample;
a tissue trapping region at a second end of the inlet channel downstream from the first end, the tissue trapping region including one or more tissue traps configured to catch a tissue sample flowing through the inlet channel such that the fluid sample contacts the one or more tissue traps;
wherein at least one of the one or more tissue traps comprises a bottom surface defined at a lower depth in the substrate than a bottom surface of the inlet channel defined in the substrate; and
one or more channels providing an outlet, the one or more channels comprising one or more suction channels downstream from the one or more tissue traps and configured to hold the tissue sample in place within the lower depth of the one or more tissue traps.

16. The microfluidic device of claim 15 , wherein at least one of the one or more tissue traps comprises an arrangement of one or more walls.

17. The microfluidic device of claim 15 , wherein the one or more channels providing the outlet comprise one or more branch channels connecting to the second end of the inlet channel where the second end of the inlet channel and the tissue trapping region converge.

18. The microfluidic device of claim 17 , wherein the convergence of the second end of the inlet channel and the tissue trapping region further comprises:
a first branch channel coupled to the second end of the inlet channel at a first junction and configured to direct a first portion of the fluid sample in a first direction; and
a second branch channel coupled to the second end of the inlet channel at the first junction and configured to direct a second portion of the fluid sample in a second direction, different from the first direction, wherein at least one of the one or more tissue traps is positioned at the first junction.

19. The microfluidic device of claim 18 , wherein the first branch channel and the second branch channel converge at a second junction downstream from the one or more tissue traps.

20. The microfluidic device of claim 18 , further comprising:
a first suction channel coupling at least one of the one or more tissue traps to the first branch channel at a third junction downstream from the second end of the inlet channel; and
a second suction channel coupling the at least one of the one or more tissue traps to the second branch channel at a fourth junction downstream from the second end of the inlet channel.

21. The microfluidic device of claim 18 , wherein a diameter of at least one of the one or more tissue traps is about twice that of the inlet channel.

22. The microfluidic device of claim 15 , further comprising an inlet port coupled to the first end of the inlet channel and configured to deliver the fluid sample to the inlet channel.

23. The microfluidic device of claim 22 , further comprising a bubble trapping structure coupled to the inlet channel downstream from the inlet port, the bubble trapping structure configured to facilitate evacuation of air bubbles from the fluid sample.

24. The microfluidic device of claim 22 , wherein the inlet port comprises a first threaded connector configured for attachment to a fluid line.

25. The microfluidic device of claim 23 , wherein a surface of the bubble trapping structure has a shape defined in part by a parabolic function.

26. The microfluidic device of claim 23 , wherein the bubble trapping structure further comprises a second threaded connector configured for attachment to an air release line.

27. The microfluidic device of claim 15 , wherein the substrate is formed from a biocompatible material.

28. The micr

Assignees

• Charles Stark Draper Laboratory Inc
• Massachusetts Inst Technology

Inventors

• Beckwith Ashley Lynne
• Borenstein Jeffrey
• Moore Nathan
• Doty Daniel
• Velasquez-Garcia Luis

Classifications

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L2400/0463

  Hydrodynamic forces, venturi nozzles · CPC title

• B01L2200/0684

  Venting, avoiding backpressure, avoid gas bubbles · CPC title

• G01N33/5082

  Supracellular entities, e.g. tissue, organisms · CPC title

• B01L2200/027

  for microfluidic devices · CPC title