Compositions and Methods for Clearing a Biological Sample
US-2017227430-A1 · Aug 10, 2017 · US
Composition for biological tissue transparency and method for biological tissue transparency using same
US11022528B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11022528-B2 |
| Application number | US-201816604113-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 23, 2018 |
| Priority date | Apr 21, 2017 |
| Publication date | Jun 1, 2021 |
| Grant date | Jun 1, 2021 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The clarity method for biotissue using a biotissue clearing agent including CHAPS of the present invention enables fast tissue clarity without denaturation.
First claim
Opening claim text (preview).
What is claimed is: 1. A clarity method for clearing fixed biotissue, comprising the steps of: a) pretreating the fixed biotissue with a solution comprising an effective amount of a saccharide; and b) clearing the pretreated biotissue by contacting the pretreated biotissue with a composition comprising an effective amount of the compound represented by formula 1 below, or the hydrate of the same: 2. The clarity method for biotissue according to claim 1 , wherein the saccharide is one or more materials selected from the group consisting of monosaccharides, disaccharides, and polysaccharides. 3. The clarity method for biotissue according to claim 2 , wherein the monosaccharide is fructose, galactose, glucose, or mannose; the disaccharide is sucrose, lactose, maltose, trehalose, turanose, or cellobiose; and the polysaccharide is dextran, diethylamino ethyl-dextran, dextrin, cellulose, or β-glucans. 4. The clarity method for biotissue according to claim 1 , wherein the saccharide solution is an aqueous solution containing a saccharide. 5. The clarity method for biotissue according to claim 1 , wherein the saccharide concentration in the saccharide solution is 10-70 w/v %. 6. The clarity method for biotissue according to claim 2 , wherein saccharide is a disaccharide. 7. The clarity method for biotissue according to claim 6 , wherein the disaccharide is sucrose.
Assignees
Inventors
Classifications
Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title
substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol · CPC title
Disaccharides · CPC title
Fixative compositions · CPC title
not covered by C07J41/0005 · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US11022528B2 cover?
- The clarity method for biotissue using a biotissue clearing agent including CHAPS of the present invention enables fast tissue clarity without denaturation.
- Who is the assignee on this patent?
- Korea Res Inst Chemical Tech
- What technology area does this patent fall under?
- Primary CPC classification G01N1/34. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Jun 01 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).