Apparatus and method for manufacturing channel-coupled scaffold

Patent metadata

Field	Value
Publication number	US-11021586-B2
Application number	US-201715438215-A
Country	US
Kind code	B2
Filing date	Feb 21, 2017
Priority date	Mar 3, 2016
Publication date	Jun 1, 2021
Grant date	Jun 1, 2021

Abstract

The present disclosure relates to an apparatus and a method for manufacturing a channel-coupled scaffold. The present disclosure provides a method for manufacturing a channel-coupled scaffold, which includes: (1) a step of compressing a first elastic substrate which includes a groove on the surface of the substrate to close the groove; (2) a step of loading a scaffold composition onto the closed groove; and (3) a step of restoring the elastic substrate. The present disclosure also provides an apparatus for manufacturing a channel-coupled scaffold, which includes: a first elastic substrate which includes a groove on the surface of the substrate and onto which a scaffold composition is loaded: and a compression module which compresses the width of the groove of the elastic substrate to close it. The apparatus or method may accumulate a microchannel controlling local mass transfer, and align a collagen fiber in the scaffold at the same time.

First claim

What is claimed is:

1. A method for manufacturing a channel-coupled scaffold using an elastic substrate having a facing surface that includes a groove and a non-groove portion, with the groove having an interior defined by at least two edges separated from each other in the facing surface corresponding to the groove being in an open state, the method comprising:
applying a compressing force to a surface of the elastic substrate, the application of the compressing force forcing the elastic substrate into a compressed state that has the at least two edges, due to the applied compressing force, being in mutual contact so the groove is in a closed state;
loading a scaffold composition onto the facing surface of the elastic substrate in the compressed state, and into contact with the non-groove portion and the groove that has the at least two edges being in the mutual contact; and
maintaining the compressed state of the elastic substrate onto which the scaffold composition has been loaded, wherein the loaded scaffold composition is cured partially during the maintaining the compressed state of the elastic substance; and then
ceasing the application of the force to the elastic substrate, resulting in the at least two edges not being in mutual contact and the groove thereby being in the open state; and
forming the loaded scaffold composition into a polymer scaffold, the formed polymer scaffold covering the at least two edges across the groove in the open state;
wherein, in the loading of the scaffold composition, the scaffold composition is not loaded into the interior of the groove in the closed state, and
wherein the groove forms a channel adjoining the formed polymer scaffold.

2. The method according to claim 1 , further comprising coating the facing surface of the elastic substrate with an adhesive prior to the applying of the compressing force.

3. The method according to claim 2 , wherein the adhesive is one or more selected from a group consisting of glutaraldehyde, polyethyleneimine, poly-L-lysine, poly-D-lysine and polydopamine.

4. The method according to claim 1 , wherein the maintaining of the compressed state includes maintaining the compressed state of the elastic substrate for 1-30 minutes.

5. The method according to claim 1 , wherein the applying of the compressing force further comprises applying the compressing force in a direction perpendicular to the length of the groove.

6. The method according to claim 1 , wherein, by the application of the compressing force, the elastic substrate becomes compressed by 5-80% of its original width before the elastic substrate becomes compressed along a direction perpendicular to the groove.

7. The method according to claim 1 , further comprising forming a concentration gradient of diagnostic or therapeutic material in the formed polymer scaffold by transporting the diagnostic or therapeutic material through the channel to contact the formed polymer scaffold.

Assignees

• Korea Inst Sci & Tech

Inventors

• Choi Nakwon
• Kim Sohyun
• JEONG Sohyeon
• Yoon Eui Sung
• Lee Changjoon Justin
• Cho Il-Joo

Classifications

• C12N2533/30

  Synthetic polymers · CPC title

• C08J2465/00

  Characterised by the use of macromolecular compounds obtained by reactions forming a carbon-to-carbon link in the main chain (C08J2407/00 - C08J2457/00, C08J2461/00 take precedence); Derivatives of such polymers · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• B29K2995/0046

  Elastic · CPC title

• C08J7/0427

  with only one layer of a composition containing a polymer binder (with more layers C08J7/042) · CPC title