Compositions for neuromodulation of mosquito feeding behavior and uses thereof

Patent metadata

Field	Value
Publication number	US-11020496-B2
Application number	US-201816127587-A
Country	US
Kind code	B2
Filing date	Sep 11, 2018
Priority date	Sep 11, 2017
Publication date	Jun 1, 2021
Grant date	Jun 1, 2021

Abstract

The present disclosure relates to compositions and methods for enhancing mosquito feeding.

First claim

We claim:

1. A composition comprising:
(a) a mosquito food source, wherein the mosquito food source is sucrose; and
(b) a mosquito neuromodulation agent;
wherein the mosquito neuromodulation agent enhances mosquito feeding in comparison to a control composition without the mosquito neuromodulation agent; and
wherein the mosquito neuromodulation agent is selected from TCB-2, polygodial, antagonist G, or a combination thereof.

2. The composition of claim 1 , wherein the sucrose is present at about 10% sucrose.

3. The composition of claim 1 , further comprising 15 mM NaCl, 1 mM NaHCO 3 , 1 μM MgCl 2 , 5 μM β,γ-methyleneadenosine 5′-triphosphate disodium salt (β,γ-met ATP), or a combination thereof.

4. The composition of claim 1 , wherein the mosquito neuromodulation agent comprises TCB-2.

5. The composition of claim 1 , wherein the mosquito neuromodulation agent comprises polygodial.

6. The composition of claim 1 , wherein the mosquito neuromodulation agent comprises antagonist G.

7. A method for enhancing mosquito feeding, comprising:
(a) providing a payload reservoir containing a composition, wherein the composition comprises:
a mosquito food source, wherein the mosquito food source is sucrose; and
a mosquito neuromodulation agent; and

(b) allowing mosquitoes to alight on or feed on the payload reservoir;
wherein the mosquito neuromodulation agent enhances mosquito feeding in comparison to a control composition without the mosquito neuromodulation agent; and
wherein the mosquito neuromodulation agent is selected from TCB-2, polygodial, antagonist G, or a combination thereof.

8. The method of claim 7 , wherein the sucrose is present at about 10% sucrose.

9. The method of claim 7 , wherein the composition further comprises 15 mM NaCl, 1 mM NaHCO 3 , 1 μM MgCl 2 , 5 μM β,γ-methyleneadenosine 5′-triphosphate disodium salt (β,γ-met ATP), or a combination thereof.

10. The method of claim 7 , wherein the composition further comprises a detector conjugate, wherein the detector conjugate comprises a gold nanoparticle conjugated to a specific detector molecule.

11. The method of claim 7 , wherein the mosquito neuromodulation agent enhances mosquito feeding by at least 10%.

12. The method of claim 7 , wherein the mosquitoes are Aedes aegypti.

13. The composition of claim 1 , wherein the mosquito neuromodulation agent enhances mosquito feeding by at least 10%.

14. The method of claim 7 , wherein the mosquito neuromodulation agent comprises TCB-2.

15. The method of claim 7 , wherein the mosquito neuromodulation agent comprises polygodial.

16. The method of claim 7 , wherein the mosquito neuromodulation agent comprises antagonist G.

17. The composition of claim 1 , wherein the composition further comprises a detector conjugate, wherein the detector conjugate comprises a gold nanoparticle conjugated to a specific detector molecule.

Assignees

• Univ Central Florida Res Found Inc

Inventors

• Willenberg Bradley Jay
• Seal Sudipta
• Bosak Alexander John
• Willenberg Alicia Renae

Classifications

• A61K31/4168 Primary

  having a nitrogen attached in position 2, e.g. clonidine · CPC title

• A61K49/0004

  Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions · CPC title

• A61K49/0008

  Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure · CPC title

• A61K49/0093 Primary

  Nanoparticle, nanocapsule, nanobubble, nanosphere, nanobead, i.e. having a size or diameter smaller than 1 micrometer, e.g. polymeric nanoparticle · CPC title

• A61K31/11

  Aldehydes · CPC title