Wheat MS1 polynucleotides, polypeptides, and methods of use

Patent metadata

Field	Value
Publication number	US-11015209-B2
Application number	US-201515514195-A
Country	US
Kind code	B2
Filing date	Sep 21, 2015
Priority date	Sep 26, 2014
Publication date	May 25, 2021
Grant date	May 25, 2021

Abstract

Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

First claim

That which is claimed:

1. A method of restoring fertility to a male-sterile wheat plant, the method comprising:
introducing into a wheat plant that is homozygous recessive for a disruption of endogenous MS1 polynucleotide, wherein the disruption results in male-sterility, wherein the endogenous MS1 polynucleotide is selected from the group consisting of:
a. a polynucleotide that encodes the polypeptide of SEQ ID NO:5;
b. a polynucleotide comprising the sequence set forth in SEQ ID NO:4, 7, or 9; and
c. a polynucleotide having at least 90% sequence identity to SEQ ID NO: 4, 7, or 9;

a polynucleotide that encodes a polypeptide having at least 90% sequence identity to SEQ ID NO: 3, 5, 39 or 40, wherein the expression of the polypeptide restores male fertility to the male-sterile wheat plant, wherein the polynucleotide that encodes the male fertility restoration polypeptide is operably linked to a polynucleotide of interest encoding a reporter or marker product.

2. The method of claim 1 , wherein the polypeptide that restores the wheat plant's male fertility is from wheat or barley.

3. The method of claim 1 , wherein the polynucleotide that encodes the polypeptide that restores male fertility to the male-sterile wheat plant is driven by a heterologous promoter active in a plant cell.

4. The method of claim 1 , wherein the polynucleotide that encodes the polypeptide that restores male fertility to the male-sterile wheat plant is driven by a MS1 promoter.

5. The method of claim 4 , wherein the MS1 promoter is from wheat or barley.

6. The method of claim 4 , wherein the MS1 promoter is the nucleic acid sequence of SEQ ID NO: 6, SEQ ID NO:41, or the nucleotide sequence from 1-902 of SEQ ID NO:1.

7. The method of claim 1 , wherein the polynucleotide that encodes the polypeptide that restores male fertility to the male-sterile wheat plant is driven by a male tissue-preferred promoter.

8. The method of claim 1 , wherein the polynucleotide of interest encodes a reporter that confers antibiotic resistance or herbicide resistance.

9. The method of claim 1 , wherein the polynucleotide of interest encodes a color marker.

10. The method of claim 1 , wherein the polynucleotide of interest is expressed in seed.

Assignees

• Pioneer Hi Bred Int

Inventors

• Albertsen Marc C
• Baumann Ute
• Cigan Andrew Mark
• Singh Manjit
• Tucker Elise
• Whitford Ryan

Classifications

• A01H1/022

  Genic fertility modification, e.g. apomixis · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C07K14/415

  from plants · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12N15/8213

  Targeted insertion of genes into the plant genome by homologous recombination · CPC title