Single-use cell culturing apparatus and culturing bag

Patent metadata

Field	Value
Publication number	US-11015159-B2
Application number	US-201615749609-A
Country	US
Kind code	B2
Filing date	Jul 1, 2016
Priority date	Aug 7, 2015
Publication date	May 25, 2021
Grant date	May 25, 2021

Abstract

A single-use cell culturing apparatus and a culturing bag are provided, each of which can improve efficiency of stirring a culture solution and further reduce a possibility of leakage of the culture solution. The single-use cell culturing apparatus of the present invention includes a housing that accommodates the culturing bag which is flexible and can seal the culture solution inside thereof. The housing has, on an inner surface thereof, a curved convex-shaped protrusion provided at a portion of a contact face at which the housing is in contact with the culturing bag. The culturing bag of the present invention is accommodated in the housing of the single-use cell culturing apparatus; is flexible; and can seal the culture solution inside thereof. The culturing bag includes a concave portion having a shape corresponding to the curved convex protrusion disposed on a part of the housing.

First claim

The invention claimed is:

1. A single-use cell culturing apparatus, comprising:
a housing that has a cylindrical space with a center axis which is fixed in a vertical direction and configured to accommodate a cylindrical culturing bag which is flexible and can seal a culture solution inside thereof, the housing rotatable in a horizontal plane around an axis that is in an eccentric position from the center axis of the housing to stir the culture solution inside the bag by centrifugal force of rotation of the housing; and
a curved convex-shaped protrusion provided on an inner sidewall surface of the housing at a position at which the housing comes in contact with the culturing bag and which corresponds to a location below a liquid level of the culture solution in the culturing bag.

2. The single-use cell culturing apparatus according to claim 1 ,
wherein the culturing bag is accommodated in the housing.

3. The single-use cell culturing apparatus according to claim 1 ,
wherein a W/D is within a range from 0.05 to 0.15, in which W is a height of the protrusion and D is an internal dimension of the housing.

4. The single-use cell culturing apparatus according to claim 1 ,
wherein the protrusion is provided on the inner sidewall surface of the housing in a direction parallel to the vertical direction or at a prescribed tilt angle with respect to the vertical direction.

5. The single-use cell culturing apparatus according to claim 1 , further comprising:
another protrusion disposed on an inner bottom surface of the housing.

6. The single-use cell culturing apparatus according to claim 1 ,
wherein the inner sidewall surface of the housing has a plurality of holes, and
wherein the protrusion includes a fixing part which is mountable in a plurality of positions and a plurality of angles to the holes of the inner sidewall surface of the housing and detachable therefrom.

7. The single-use cell culturing apparatus according to claim 1 ,
wherein the protrusion has a substantially triangular prismatic shape or a substantially semicircular columnar shape.

8. The single-use cell culturing apparatus according to claim 1 ,
wherein a height of the protrusion is 0.5 to 5 times a length of a base of the protrusion.

9. The single-use cell culturing apparatus according to claim 1 ,
wherein a side of the protrusion is 0.05 to 0.5 times a length of a base in terms of a radius of curvature.

10. The single-use cell culturing apparatus according to claim 1 ,
wherein a length L in a longitudinal direction of the protrusion is within a range from not less than 0.05 to not more than 1 in terms of a L/H, in which H is the liquid level height of the culture solution sealed in the culturing bag.

11. The single-use cell culturing apparatus according to claim 1 , further comprising:
a support stand which supports the housing,
wherein the housing is rotatable in the horizontal plane relative to the support stand.

Assignees

• Hitachi Ltd
• Fujimori Kogyo Co Ltd

Inventors

• Nanba Masaru
• Andou Takashi
• SUGITA Yui
• Murakami Sei
• Matsuda Hiroyuki

Classifications

• C12M23/14 Primary

  Bags · CPC title

• C12M23/48

  Holding appliances; Racks; Supports (holding devices for laboratory apparatus B01L9/00) · CPC title

• C12M23/26

  flexible (flexible containers for laboratory use B01L3/505) · CPC title

• C12M27/20

  Baffles; Ribs; Ribbons; Auger vanes · CPC title

• C12M1/06 Primary

  with agitator, e.g. impeller · CPC title