Anti-aging transgenic Caenorhabditis elegans

Patent metadata

Field	Value
Publication number	US-11013221-B2
Application number	US-201716095414-A
Country	US
Kind code	B2
Filing date	Apr 21, 2017
Priority date	Apr 25, 2016
Publication date	May 25, 2021
Grant date	May 25, 2021

Abstract

The present invention relates to: a transgenic Caenorhabditis elegans in which a glutamine tRNA 5′ terminus-derived fragment (Gln 5′-tsRNA) is overexpressed; a preparation method therefor; and a method for screening for aging-associated factors by using the transgenic Caenorhabditis elegans. A transgenic Caenorhabditis elegans model provided in the present invention is an animal model in which Gln 5′-tsRNA is overexpressed such that aging is inhibited. When the model of the present invention is used, anti-aging mechanisms can be easily investigated, thereby significantly contributing to various research fields such as that of developing new anti-aging drugs and screening for age-inducing materials.

First claim

The invention claimed is:

1. A transgenic Caenorhabditis elegans ( C. elegans ) whose genome comprises a nucleic acid encoding glutamine tRNA 5′, wherein said C. elegans has an increased lifespan compared to a wild-type C. elegans and where said nucleic acid consists of the sequence set forth in SEQ ID NO: 1.

2. The transgenic C. elegans of claim 1 , which is for an anti-aging animal model.

3. The transgenic C. elegans of claim 1 , which exhibits an anti-aging effect due to mitochondrial activation.

4. The transgenic C. elegans of claim 1 , which exhibits an anti-aging effect via dietary restriction (DR)/AMP-dependent kinase (AMPK) pathways.

5. A method of producing the transgenic C. elegans of claim 1 , the method comprising:
(a) constructing a recombinant vector including a U6 promoter, Gln 5′-tsRNA DNA and an U6 3′ untranslated region (UTR); and
(b) injecting the recombinant vector into C. elegans.

6. The method of claim 5 , wherein the injection in step (b) is done by microinjection.

Assignees

• Postech Acad Ind Found
• Inst Basic Science
• Daegu Gyeongbuk Inst Of Science

Inventors

• Nam Hong Gil
• Jung Gyoo Yeol
• Lee Seung Jae
• Hwang Woo Seon
• Shin Gi Won
• Seo Mi Hwa

Classifications

• A61K49/0008 Primary

  Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure · CPC title

• A01K67/64 Primary

  Genetically modified nematodes · CPC title

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title

• A01K67/027 Primary

  New or modified breeds of vertebrates · CPC title

• G01N33/50

  Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing (measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q) · CPC title