Method for producing mogrol or mogrol glycoside

Patent metadata

Field	Value
Publication number	US-11008600-B2
Application number	US-201716318268-A
Country	US
Kind code	B2
Filing date	Jul 18, 2017
Priority date	Jul 19, 2016
Publication date	May 18, 2021
Grant date	May 18, 2021

Abstract

A novel method for producing a mogrol glycoside and mogrol has been required. The present invention provides a method for producing a mogrol glycoside and/or mogrol, said method comprising a step for cleaving at least one glucoside bond in a mogrol glycoside.

First claim

The invention claimed is:

1. A method of producing mogrol and/or a mogrol glycoside comprising reacting a protein selected from the group consisting of proteins (a) to (c) shown below with a substrate mogrol glycoside, under reaction conditions for hydrolyzing at least one glucoside bond of said substrate mogrol glycoside, thereby producing the mogrol and/or mogrol glycoside product:
(a) a protein consisting of the amino acid sequence of SEQ ID NO: 2;
(b) a protein consisting of an amino acid sequence wherein 1 to 83 amino acids have been deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 2, and having an activity to hydrolyze at least one glucoside bond of the substrate mogrol glycoside; and
(c) a protein having an amino acid sequence having a sequence identity of 90% or more to the amino acid sequence of SEQ ID NO: 2, and having an activity to hydrolyze at least one glucoside bond of the substrate mogrol glycoside.

2. The method according to claim 1 , wherein the substrate mogrol glycoside to be reacted with the protein is at least one member selected from the group consisting of mogroside V, mogroside IV, siamenoside I, 11-oxomogroside, mogroside I, mogroside IVA, mogroside III, mogroside IIIA1, mogroside IIIA2, mogroside IIIE, mogroside IIA, mogroside IIA1, mogroside IIA2, mogroside IIB, mogroside IIE, mogroside IA1, and mogroside IE1.

3. The method according to claim 2 , wherein the substrate mogrol glycoside is at least one member selected from the group consisting of mogroside V, mogroside IIIE, and siamenoside I.

4. The method according to claim 3 , wherein the substrate mogrol glycoside is mogroside V.

5. The method according to claim 1 , wherein the at least one glucoside bond is any of a β-1,6-glucoside bond of gentiobiose added to position 3 of mogrol, a glucoside bond between glucose added to position 3 of mogrol and aglycone, mogrol, a β-1,6-glucoside bond of a branched trisaccharide added to position 24 of mogrol, a β-1,6-glucoside bond of gentiobiose added to position 24 of mogrol, a β-1,2-glucoside bond of sophorose added to position 24 of mogrol, and/or a glucoside bond between glucose added to position 24 of mogrol and aglycone, mogrol.

6. A method of producing mogrol and/or a mogrol glycoside comprising contacting an enzyme agent from a non-human transformed cell obtained by introducing, into a host cell, a polynucleotide selected from the group consisting of polynucleotides (a) to (d) shown below, with a substrate mogrol glycoside, under reaction conditions for hydrolyzing at least one glucoside bond of said substrate mogrol glycoside, thereby producing the mogrol and/or mogrol glycoside product:
(a) a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 1;
(b) a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2;
(c) a polynucleotide encoding a protein consisting of an amino acid sequence wherein 1 to 83 amino acids have been deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 2, and having an activity to hydrolyze at least one glucoside bond of the substrate mogrol glycoside; and
(d) a polynucleotide encoding a protein having an amino acid sequence having a sequence identity of 90% or more to the amino acid sequence of SEQ ID NO: 2, and having an activity to hydrolyze at least one glucoside bond of the substrate mogrol glycoside.

7. The method according to claim 6 , wherein the polynucleotide is inserted into an expression vector.

8. The method according to claim 6 , wherein the non-human transformed cell is a transformed koji mold, a transformed yeast, a transformed bacterium, or a transformed plant.

9. The method according to claim 6 , wherein the substrate mogrol glycoside to be contacted with the enzyme agent is at least one member selected from the group consisting of mogroside V, mogroside IV, siamenoside I, 11-oxomogroside, mogroside I, mogroside IVA, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IIA, mogroside IIA 1 , mogroside IIA 2 , mogroside IIB, mogroside IIE, mogroside IA 1 , and mogroside IE 1 .

10. The method according to claim 9 , wherein the substrate mogrol glycoside is at least one member selected from the group consisting of mogroside V, mogroside IIIE, and siamenoside I.

11. The method according to claim 6 , wherein the at least one glucoside bond is any of a β-1,6-glucoside bond of gentiobiose added to position 3 of mogrol, a glucoside bond between glucose added to position 3 of mogrol and aglycone, mogrol, a β-1,6-glucoside bond of a branched trisaccharide added to position 24 of mogrol, a β-1,6-glucoside bond of gentiobiose added to position 24 of mogrol, a β-1,2-glucoside bond of sophorose added to position 24 of mogrol, and/or a glucoside bond between glucose added to position 24 of mogrol and aglycone, mogrol.

12. A method of producing mogrol and/or a mogrol glycoside comprising culturing a non-human transformant in which a polynucleotide selected from the group consisting of polynucleotides (a) to (d) shown below is introduced, and contacting an enzyme obtained from said cultured non-human transformant with a substrate mogrol glycoside under reaction conditions for hydrolyzing at least one glucoside bond of said substrate mogrol glycoside, thereby producing the mogrol and/or mogrol glycoside product:
(a) a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 1;
(b) a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2;
(c) a polynucleotide encoding a protein consisting of an amino acid sequence wherein 1 to 83 amino acids have been deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 2, and having an activity to hydrolyze at least one glucoside bond of the substrate mogrol glycoside; and
(d) a polynucleotide encoding a protein having an amino acid sequence having a sequence identity 90% or more to the amino acid sequence of SEQ ID NO: 2, and having an activity to hydrolyze at least one glucoside bond of the substrate mogrol glycoside.

13. The method according to claim 12 , wherein the polynucleotide is inserted into an expression vector.

14. The method according to claim 12 , wherein the non-human transformant is a transformed koji mold, a transformed yeast, a transformed bacterium or a transformed plant.

15. The method according to claim 12 , wherein the at least one glucoside bond is any of a β-1,6-glucoside bond of gentiobiose added to position 3 of mogrol, a glucoside bond between glucose added to position 3 of mogrol and aglycone, mogrol, a β-1,6-glucoside bond of a branched trisaccharide added to position 24 of mogrol, a β-1,6-glucoside bond of gentiobiose added to position 24 of mogrol, a β-1,2-glucoside bond of sophorose added to position 24 of mogrol, and/or a glucoside bond between glucose added to position 24 of mogrol and aglycone, mogrol.

Assignees

• Suntory Holdings Ltd

Inventors

• Ochiai Misa
• Ono Eiichiro

Classifications

• C12Y302/01021 Primary

  Beta-glucosidase (3.2.1.21) · CPC title

• C12P19/44

  Preparation of O-glycosides, e.g. glucosides {(polysaccharides and not substituted disaccharides C12P19/04, C12P19/12)} · CPC title

• C12N5/10 Primary

  Cells modified by introduction of foreign genetic material · CPC title

• C12N15/09

  Recombinant DNA-technology · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title