Method for optimizing a process for freeze drying a bacteria-containing concentrate
US-2015232801-A1 · Aug 20, 2015 · US
US11005089B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11005089-B2 |
| Application number | US-201615243614-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 22, 2016 |
| Priority date | Aug 22, 2016 |
| Publication date | May 11, 2021 |
| Grant date | May 11, 2021 |
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A method of preparing a porous sheet includes mixing a matrix material dispersion including a matrix material dispersed in a first dispersion medium with a microorganism dispersion including microorganisms in a second dispersion medium, to form a mixture. The first and the second dispersion media are removed from the mixture to form a matrix sheet, and the microorganisms are decomposed from the matrix sheet to form the porous sheet.
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What is claimed is: 1. A method of preparing a porous sheet, comprising: mixing a matrix material dispersion comprising a matrix material dispersed in a first dispersion medium with a microorganism dispersion comprising microorganisms in a second dispersion medium, to form a mixture; removing the first and the second dispersion media from the mixture to form a matrix sheet that includes spaces filled by the microorganisms; and decomposing the microorganisms from the matrix sheet to form pores in the porous sheet that correspond to the spaces, wherein the decomposing includes both chemical and thermal treatments and freeze drying the matrix sheet prior to the thermal treatment, wherein porosity and pore shape of the matrix sheet is dependent upon microorganism species and concentration. 2. The method of claim 1 , wherein the matrix material includes carbon. 3. The method of claim 1 , wherein the microorganisms include bacteria. 4. The method of claim 1 , wherein removing the first and the second dispersion media includes at least one of filtration and evaporation. 5. The method of claim 1 , wherein the microorganisms have a dimension of about 0.1 micrometers to about 50 micrometers. 6. The method of claim 1 , wherein the microorganisms have a dimension of about 0.1 micrometers to about 5 micrometers. 7. The method of claim 1 , wherein the microorganisms contain more than one species of microorganism. 8. The method of claim 1 , wherein the first dispersion medium and the second dispersion medium are different. 9. The method of claim 1 , wherein chemically treating the matrix sheet comprises applying a bleach treatment to the matrix sheet.
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