In situ chemical sensing electrode reconditioning

Patent metadata

Field	Value
Publication number	US-10996185-B2
Application number	US-201715601503-A
Country	US
Kind code	B2
Filing date	May 22, 2017
Priority date	May 23, 2016
Publication date	May 4, 2021
Grant date	May 4, 2021

Abstract

A cycle of positive and negative voltage pulses applied to an electrode sensor removes passivation of an electrode surface. The conditioned sensors have improved sensitivity to concentrations for analytes of interest. The electrode surfaces can also be passivated on purpose to reduce sensitivity. The voltages applied are varied according to the solution present.

First claim

What is claimed is:

1. A method comprising:
providing a sensor in a liquid, the sensor comprising at least one electrode having a surface in contact with the liquid, and a platinum reference electrode;
applying a conditioning voltage to the at least one electrode to remove a passivation layer from said surface, wherein the conditioning voltage is a pulse of DC voltage with controlled DC voltage amplitude and controlled pulse length, and
maintaining a passivation layer on a surface of the platinum reference electrode by applying a passivating voltage to the platinum reference electrode, thereby providing a more stable platinum reference electrode,
wherein the passivating voltage is a pulse of DC voltage with controlled DC voltage amplitude and controlled pulse length, and
wherein the controlled DC voltage amplitude of the conditioning voltage is in a range between −1.0 to 0.6 volts with respect to the platinum reference electrode.

2. The method of claim 1 , wherein the pulse length of the conditioning voltage lasts for 5 seconds.

3. The method of claim 1 , further comprising applying a measuring pulse of 0 to 0.6 volts with respect to the platinum reference electrode to the at least one electrode to measure a concentration of a target analyte in the liquid.

4. The method of claim 3 , wherein the applying the conditioning voltage followed by the applying the measuring pulse is carried out repeatedly in a cycle.

5. The method of claim 4 , wherein the cycle comprises at least 10 repetitions.

6. The method of claim 1 , wherein the at least one electrode has been previously conditioned.

7. The method of claim 3 , wherein the liquid is phosphate-buffered saline or ultra-pure water, and the target analyte is glucose or hydrogen peroxide.

8. The method of claim 7 , wherein the at least one electrode is coated with an enzyme.

9. The method of claim 8 , wherein the enzyme is glucose oxidase.

10. A method comprising:
providing a sensor in a liquid, the sensor having at least one electrode having a surface in contact with the liquid and at least one platinum reference electrode that is maintained in a passivated state;
applying a conditioning voltage to the at least one electrode to remove a passivation layer from said surface, wherein the conditioning voltage is a pulse of DC voltage with controlled DC voltage amplitude and controlled pulse length;
applying a measuring voltage pulse to the at least one electrode, subsequently to the conditioning voltage, to measure a concentration of a target analyte in the liquid, and
maintaining a passivation layer on a surface of the platinum reference electrode by applying a passivating voltage to the platinum reference electrode, thereby maintaining the passivated state,
wherein the passivating voltage is a pulse of DC voltage with controlled DC voltage amplitude and controlled pulse length, and
wherein the DC voltage amplitude of the conditioning voltage is negative with respect to the platinum reference electrode, and the measuring voltage pulse is positive with respect to the platinum reference electrode.

11. The method of claim 10 , wherein the DC voltage amplitude of the conditioning voltage is −1.0 to 0.6 volts with respect to the platinum reference electrode and the measuring voltage pulse is 0 to 0.6 volts with respect to the platinum reference electrode.

12. The method of claim 11 , wherein the pulse length of the conditioning voltage and a pulse length of the measuring voltage pulse are each 5 seconds long.

13. The method of claim 12 , wherein the applying of the conditioning voltage followed by the applying of the measuring voltage pulse is repeated at least 10 times.

14. The method of claim 10 , wherein the at least one electrode has been previously conditioned.

15. The method of claim 10 , wherein the liquid is phosphate-buffered saline or ultra-pure water, and the target analyte is glucose or hydrogen peroxide.

16. The method of claim 15 , wherein the at least one electrode is coated with an enzyme.

17. The method of claim 16 , wherein the enzyme is glucose oxidase.

18. A method comprising:
providing a sensor in a liquid, the sensor comprising at least one electrode and at least one platinum reference electrode; and
applying a passivating voltage to the at least one platinum reference electrode to generate a passivation layer on a surface of the at least one electrode, wherein the passivating voltage is a pulse of DC voltage with controlled DC voltage amplitude and controlled pulse length;
interspersing the applying with measurement cycles of the terminal sensor, the measurement cycles to measure a concentration of a target analyte in the liquid; and
based on the interspersing, maintaining the passivation layer and obtaining more stable measurement results,
wherein the DC voltage of the passivating voltage is positive with respect to a voltage at the platinum reference electrode.

Assignees

• California Inst Of Techn

Inventors

• Chen Samson
• Scherer Axel
• Jilani Muhammad M

Classifications

• G01N27/30

  Electrodes, e.g. test electrodes; Half-cells (G01N27/414 takes precedence) · CPC title

• G01N27/3274 Primary

  Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration (coding of calibration information G01N33/48771) · CPC title