Device and method for isolating a target from a biological sample

Patent metadata

Field	Value
Publication number	US-10996149-B2
Application number	US-201715691105-A
Country	US
Kind code	B2
Filing date	Aug 30, 2017
Priority date	Jan 9, 2013
Publication date	May 4, 2021
Grant date	May 4, 2021

Abstract

A device and a method for isolating a target from a biological sample are provided. The target is bound to solid phase substrate to form target bound solid phase substrate. The device includes a lower plate with an upper surface having a plurality of regions. The biological sample is receivable on a first of the regions. An upper plate has a lower surface directed to the upper surface of the lower plate. A force is positioned adjacent the upper plate and attracts the target bound solid phase substrate toward the lower surface of the upper plate. At least one of the upper plate and the lower plate is movable from a first position wherein the target bound solid phase substrate in the biological sample are drawn to the lower surface of the upper plate and a second position wherein the target bound solid phase substrate are isolated from the biological sample.

First claim

We claim:

1. A device for isolating a target from a biological sample, the target being bound to solid phase substrate to form target bound solid phase substrate having a dimension, comprising:
a lower plate lying in a first plane and including a generally flat, upper surface having a plurality of regions, the biological sample receivable on a first of the regions and defining a height;
an upper plate lying in a second plane and having a lower surface directed towards the upper surface of the lower plate so as to define a gap therebetween, the lower surface of the upper plate being hydrophobic and being generally parallel to the upper surface of the lower plate; and
a magnetic force generator configured to generate a magnetic force adjacent the upper plate, the magnetic force attracting and drawing the target bound solid phase substrate in the biological sample received on the first of the regions in an upwardly direction to a location toward the lower surface of the upper plate in the gap;

wherein:
at least one of the upper plate and the lower plate is movable along a corresponding one of the first and second planes relative to the other of the at least one of the upper plate and the lower plate from a first position wherein the magnetic forces draws the target bound solid phase substrate in the biological sample to the lower surface of the upper plate and a second position wherein the target bound solid phase substrate are isolated from the biological sample; and
the gap between the lower surface of the upper plate and the upper surface of the lower plate is spaced by a dimension, wherein the dimension of the gap is greater than the dimension of the target bound solid phase substrate and less than the height of the sample so as to allow the target bound solid phase substrate to be retained in the gap as the at least one of the upper plate and the lower plate is moved from the first position to the second position.

2. The device of claim 1 wherein the regions of the lower plate are hydrophilic.

3. The device of claim 1 wherein portions of the upper surface of the lower plate outside of the regions of the lower plate are hydrophobic.

4. The device of claim 1 wherein the upper plate is movable along an axis between the first and second positions.

5. The device of claim 1 wherein the upper plate is rotatable between the first and second positions.

6. The device of claim 1 wherein the upper surface of the lower plate and lower surface of the upper surface are spaced by a predetermined distance.

7. A device for isolating a target from a biological sample, the target being bound to solid phase substrate to form target bound solid phase substrate having a dimension, comprising:
a lower plate including an upper surface having a plurality of regions, the biological sample receivable at a first of the regions and defining a height;
an upper plate having a lower surface directed to the upper surface of the lower plate so as to define a gap therebetween, the lower surface of the upper plate being hydrophobic; and
a magnetic force generator configured to generate a magnetic force adjacent the upper plate, the magnetic force attracting and drawing the target bound solid phase substrate in the biological sample received at the first of the regions in an upwardly direction to a location toward the lower surface of the upper plate in the gap;

wherein:
at least one of the upper plate and the lower plate is movable laterally relative to the other of the at least one of the upper plate and the lower plate from a first position wherein the magnetic forces draws the target bound solid phase substrate in the biological sample to the lower surface of the upper plate in the gap and a second position wherein the target bound solid phase substrate are isolated from the biological sample; and
the gap between the lower surface of the upper plate and the upper surface of the lower plate is spaced by a dimension, wherein the dimension of the gap is greater than the dimension of the target bound solid phase substrate and less than the height of the sample so as to allow the target bound solid phase substrate to be retained in the gap as the at least one of the upper plate and the lower plate is moved from the first position to the second position.

8. The device of claim 7 wherein each of the plurality of regions in the lower plate is defined by a corresponding well in the upper surface thereof.

9. The device of claim 7 wherein the lower surface of the upper plate is convex.

10. The device of claim 7 wherein the lower surface of the upper plate has a flat portion directed at the upper surface of the lower plate.

11. The device of claim 7 wherein the at least one of the upper plate and the lower plate is movable along an axis from the first position to the second position.

12. The device of claim 7 wherein the at least one of the upper plate and the lower plate is rotatable between the first and second positions.

13. The device of claim 7 wherein the upper surface of the lower plate and lower surface of the upper surface are spaced by a predetermined distance.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Beebe David J
• Casavant Ben P
• Guckenberger David J
• Berry Scott M

Classifications

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• G01N1/34 Primary

  Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)} · CPC title

• B01L2400/065

  sliding valves · CPC title

• B01L3/502792

  for moving individual droplets on a plate, e.g. by locally altering surface tension · CPC title

• C12N15/1013

  by using magnetic beads · CPC title