Analytic substrate coating apparatus and method

Patent metadata

Field	Value
Publication number	US-10996148-B2
Application number	US-202016806590-A
Country	US
Kind code	B2
Filing date	Mar 2, 2020
Priority date	Apr 15, 2005
Publication date	May 4, 2021
Grant date	May 4, 2021

Abstract

An apparatus and method for producing a coated analytic substrate using a compact and portable automated instrument located in the laboratory setting at the point of use which can consistently produce one or a plurality of coated analytic substrates “on demand” for using the analytic substrate immediately after coating, preferably without a step of rinsing the coated analytic substrate before use. The apparatus preferably uses applicator cartridges having a reservoir containing the coating compositions used to form the coatings. Preferably the cartridges are removable and interchangeable to facilitate the production of individual analytic substrates having different coatings or different coating patterns. These coated analytic substrates have superior specimen adhesion characteristics due to the improved quality of the coatings applied by the coating apparatus and due to the quickness with which the coated analytic substrates can be used in the lab after production.

First claim

What is claimed is:

1. A method, comprising:
applying a coating on an analytic substrate to form a coated surface on at least a portion of the analytic substrate, wherein the coating comprises organofunctional silane; and
applying a biological specimen to the coated surface of the analytic substrate with water between the biological specimen and the analytic substrate to produce a hydrolytic area between the biological specimen and the analytic substrate wherein in situ hydrolysis of the coating is initiated by the water between the biological specimen and the analytic substrate to cause the biological specimen to bind to the analytic substrate.

2. The method of claim 1 , wherein when the biological specimen is applied to the coated analytic substrate, the organofunctional silane in the coating becomes bound by in situ hydrolysis and condensation of at least one reactive silyl group thereof to the analytic substrate, and wherein the biological specimen binds to at least one functional group of the organofunctional silane whereby the biological specimen becomes bound to the analytic substrate.

3. The method of claim 1 , wherein the analytic substrate is a microscope slide.

4. A method, comprising:
applying a coating on an analytic substrate to form a coated surface on at least a portion of the analytic substrate, wherein the coating comprises organofunctional silane; and
applying a biological specimen to the coated surface of the analytic substrate with water associated with the biological specimen between at least a portion of the biological specimen and the analytic substrate to produce a hydrolytic area between the biological specimen and the analytic substrate wherein in situ hydrolysis of the coating is initiated by the water between the biological specimen and the analytic substrate to cause the biological specimen to bind to the analytic substrate.

5. The method of claim 4 , wherein when the biological specimen is applied to the coated analytic substrate, the organofunctional silane in the coating becomes bound by in situ hydrolysis and condensation of at least one reactive silyl group thereof to the analytic substrate, and wherein the biological specimen binds to at least one functional group of the organofunctional silane whereby the biological specimen becomes bound to the analytic substrate.

6. The method of claim 4 , wherein the analytic substrate is a microscope slide.

7. A method, comprising:
applying a coating on an analytic substrate to form a coated surface on at least a portion of the analytic substrate, wherein the coating comprises organofunctional silane; and
applying a biological specimen to the coated surface of the analytic substrate with water on, around, or within the biological specimen to produce a hydrolytic area between the biological specimen and the analytic substrate wherein in situ hydrolysis of the coating is initiated by the water between the biological specimen and the analytic substrate to cause the biological specimen to bind to the analytic substrate.

8. The method of claim 7 , wherein when the biological specimen is applied to the coated analytic substrate, the organofunctional silane in the coating becomes bound by in situ hydrolysis and condensation of at least one reactive silyl group thereof to the analytic substrate, and wherein the biological specimen binds to at least one functional group of the organofunctional silane whereby the biological specimen becomes bound to the analytic substrate.

9. The method of claim 7 , wherein the analytic substrate is a microscope slide.

Assignees

• Angros Lee H

Inventors

• Angros Lee H

Classifications

• B01L3/508 Primary

  Rigid containers without fluid transport within · CPC title

• B01L2300/0822

  Slides · CPC title

• Y10T156/1798

  with liquid adhesive or adhesive activator applying means · CPC title

• B01L99/00

  Subject matter not provided for in other groups of this subclass · CPC title

• B05D1/28

  performed by transfer from the surfaces of elements carrying the liquid or other fluent material, e.g. brushes, pads, rollers · CPC title