L-tryptophan exporter and method of producing L-tryptophan using the same

Patent metadata

Field	Value
Publication number	US-10995378-B2
Application number	US-201916479749-A
Country	US
Kind code	B2
Filing date	Feb 22, 2019
Priority date	Feb 23, 2018
Publication date	May 4, 2021
Grant date	May 4, 2021

Abstract

The present disclosure relates to a microorganism producing L-tryptophan in which the microorganism is modified such that a protein having an L-tryptophan-exporting activity comprising the amino acid sequence of SEQ ID NO: 1 is expressed, and a method for producing L-tryptophan using the microorganism.

First claim

The invention claimed is:

1. A microorganism producing L-tryptophan, wherein the microorganism expresses a protein having L-tryptophan-exporting activity and comprising the amino acid sequence of SEQ ID NO: 1, and
wherein the microorganism is Corynebacterium glutamicum.

2. A microorganism producing L-tryptophan, wherein the microorganism expresses a protein having L-tryptophan-exporting activity and comprising the amino acid sequence of SEQ ID NO. 1 , and wherein the microorganism is Escherichia coli.

3. A method for producing L-tryptophan, comprising:
culturing a microorganism producing L-tryptophan in a medium, wherein the microorganism expresses a protein having L-tryptophan-exporting activity and comprising the amino acid sequence of SEQ ID NO: 1 , and wherein the microorganism is Corynebacterium glutamicum ; and
recovering the L-tryptophan from the cultured microorganism or cultured medium.

4. A method for producing L-tryptophan, comprising:
culturing a microorganism producing L-tryptophan in a medium, wherein the microorganism expresses a protein having L-tryptophan-exporting activity and comprising the amino acid sequence of SEQ ID NO: 1, wherein the microorganism is Escherichia coli , and
recovering the L-tryptophan from the cultured microorganism or cultured medium.

Assignees

• Cj Cheiljedang Corp

Inventors

• Jung Moo Young
• Seo Chang Il
• Kim Hyo Jin
• Kim Tae Yeon
• Kim Hyun Ah
• Son Sung Kwang
• Yoo Hye Ryun
• Lee Jae Min
• Cheong Ki Yong

Classifications

• C12P13/227

  Tryptophan · CPC title

• C12P13/22

  Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine · CPC title

• C12N15/70 Primary

  Vectors or expression systems specially adapted for E. coli · CPC title

• C07K14/195 Primary

  from bacteria · CPC title

• C12N15/77

  for Corynebacterium; for Brevibacterium · CPC title