Compositions and methods for islet cell transplants
US-2024269194-A1 · Aug 15, 2024 · US
Method for producing sheet-like pancreatic islet
US10995319B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10995319-B2 |
| Application number | US-201214110638-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 6, 2012 |
| Priority date | Apr 8, 2011 |
| Publication date | May 4, 2021 |
| Grant date | May 4, 2021 |
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- Title
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Abstract
Official abstract text for this publication.
The present invention provides a method of producing a sheet-like pancreatic islet, comprising culturing an isolated pancreatic islet in a culture vessel, wherein a polypeptide comprising an EC1 domain of E-cadherin and having a binding ability to said E-cadherin is fixed on or applied to a surface of a solid phase, while being adhered to the solid phase surface for a period sufficient for the pancreatic islet to take a sheet-like form.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of producing a sheet-like pancreatic islet, comprising: (i) preparing an isolated pancreatic islet of 200 microns or more in diameter without trypsin; (ii) plating the prepared isolated pancreatic islet of (i) onto a coated surface of a sold phase of a culture vessel, wherein the coated surface comprises a polypeptide comprising an EC1 domain of E-cadherin and having a binding ability to the E-cadherin fixed on or applied to the surface of the solid phase of the culture vessel, wherein the polypeptide is a fusion polypeptide comprising an extracellular domain of E-cadherin and an Fc region of immunoglobulin; and (iii) culturing the plated pancreatic islet of (ii) for about 6 days to 8 weeks to allow the isolated pancreatic islet to spread over the coated solid phase surface to produce a sheet of pancreatic islet, wherein the sheet of pancreatic islet shows a pancreatic islet function equal to or not less than that of a pancreatic islet cultured on a non-coated surface of the solid phase of the culture vessel. 2. The method according to claim 1 , wherein the plated isolated pancreatic islet is cultured for about 10 days to 8 weeks.
Assignees
Inventors
Classifications
Proteins · CPC title
Atmosphere, e.g. low oxygen conditions · CPC title
Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)} · CPC title
Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto · CPC title
- C12N5/0676Primary
Pancreatic cells · CPC title
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- What does patent US10995319B2 cover?
- The present invention provides a method of producing a sheet-like pancreatic islet, comprising culturing an isolated pancreatic islet in a culture vessel, wherein a polypeptide comprising an EC1 domain of E-cadherin and having a binding ability to said E-cadherin is fixed on or applied to a surface of a solid phase, while being adhered to the solid phase surface for a period sufficient for the …
- Who is the assignee on this patent?
- Azuma Koji, Fujita Yasutaka, Yaguchi Hiroshi, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0676. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 04 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).