Cell culturing system for cultivating adherent cells and liquid supply interface comprising a cell culture container

Patent metadata

Field	Value
Publication number	US-10995313-B2
Application number	US-201816118922-A
Country	US
Kind code	B2
Filing date	Aug 31, 2018
Priority date	Jan 23, 2013
Publication date	May 4, 2021
Grant date	May 4, 2021

Abstract

The invention relates to a liquid supply interface for a cell culture system for supplying cell cultures found in different cell culture containers with a nutrient medium, wherein the liquid supply interface comprises: a housing defining a flow area; a first connection formation for the liquid-transferring connection of a first fluid line to the housing; a second connection formation formed separately from the first for the liquid-transferring connection of a second fluid line to the housing; a third connection formation formed separately from the first two for the liquid-transferring connection of the housing to a third fluid line; a coupling formation formed separately from the connection formations, which is formed for the producible and detachable liquid-transferring coupling contact according to the operation, with a corresponding counter-coupling formation of a cell culture container.

First claim

The invention claimed is:

1. A cell culture system comprising:
at least one cell culture container for collecting and supplying adherent cells therein,
a nutrient medium reservoir,
a cleaning fluid reservoir, and
a liquid supply interface,
wherein the liquid supply interface comprises:
a housing defining a flow area;
a first connection formation for the liquid-transferring connection of a first fluid line to the housing;
a second connection formation formed separately from the first connection formation and for the liquid-transferring connection of a second fluid line with the housing;
a third connection formation formed separately from the first and second connection formations and for the liquid-transferring connection of the housing with a third fluid line;
a coupling formation formed separately from the first, second and third connection formations, which is formed for the producible and detachable liquid-transferring coupling contact with a counter-coupling formation of the at least one cell culture container;
a first liquid flow path, which extends between the flow area and the first connection formation for introducing a first liquid from outside into the flow area;
a second liquid flow path, which extends between the flow area and the second connection formation for introducing a second liquid different from the first from the outside into the flow area;
a third liquid flow path, which extends between the flow area and the third connection formation for removing a liquid from the flow area; and
a coupling flow path, which extends between the flow area and the coupling formation in order to remove a liquid from the flow area and/or to introduce it to said flow area via the coupling formation,

wherein the first, the second, and the third connection formations each have a valve configuration, which is completely surrounded, except for the respective liquid flow path, by the housing, incorporated in it, without a continuous signal- and/or power-transferring physical connection surrounded by the valve configuration up to the outside of the housing;
wherein a control configuration with a signaling means generating an electric and/or magnetic and/or electromagnetic field is assigned to each valve configuration, the field of which acts upon a valve body of the valve configuration without contact,
wherein each valve configuration can be switched off via the field, acting upon its valve body, between a blocked position, in which the valve configuration interrupts a liquid flow in the liquid flow path in which it is arranged, and an outlet position in which the valve configuration enables a liquid flow,
wherein the first connection formation connects the housing with the nutrient medium reservoir in a liquid-transferring manner and thus the first liquid flow path extends between the flow area and the nutrient medium reservoir;
wherein the second connection formation connects the housing with the cleaning fluid reservoir in a liquid-transferring manner and thus the second liquid flow path extends between the flow area and the cleaning fluid reservoir;
wherein the third connection formation connects the housing with a discharge in a liquid-transferring manner and thus the third liquid flow path extends between the flow area and the discharge;
wherein the coupling formation for coupling contact, which is producible and detachable in a liquid-transferring manner according to the operation, is formed with the counter-coupling formation of the at least one cell culture container;
wherein the first liquid is a nutrient medium;
wherein the second liquid is a cleaning fluid; and
wherein the coupling flow path is formed in order to remove the nutrient medium from the flow area and supply it to the at least one cell culture container and/or to remove it from the at least one cell culture container and introduce it to the flow area via the coupling formation, in a state coupled with the counter-coupling formation.

2. The cell culture system according to claim 1 , wherein in the flow area, a flow path passes by the valve configuration of the first connection formation, from the valve configuration of the second connection formation to the valve configuration of the third connection formation.

3. The cell culture system according to claim 2 , wherein the valve body of the valve configuration of the first connection formation and protrudes at least partially into the flow path from the valve configuration of the second connection formation to the valve configuration of the third connection formation.

4. The cell culture system according to claim 2 , wherein the counter-coupling formation of the at least one cell culture container has a container valve configuration.

5. The cell culture system according to claim 4 , wherein when the coupling contact is established between the coupling formation and the counter-coupling formation, the container valve configuration is completely surrounded by the counter-coupling formation and the housing of the liquid supply interface, with the exception of the coupling flow path, which is formed through the coupling formation and the counter-coupling formation, and does not have a continuous signal- and/or power-transferring physical connection extending from the container valve configuration up to the exterior of the counter-coupling formation or of the housing of the liquid supply interface.

6. The cell culture system according to claim 4 , wherein the container valve configuration can be switched between a blocked position and an outlet position.

7. The cell culture system according to claim 4 , wherein a flow path, in the flow area, passes by the container valve configuration from the valve configuration of the second connection formation to the valve configuration of the third connection formation when coupling contact is established between the coupling formation of the liquid supply interface and the counter-coupling formation of the at least one cell culture container.

8. The cell culture system according to claim 7 , wherein a valve body of the container valve configuration protrudes at least partially into the flow path from the valve configuration of the second connection formation to the valve configuration of the third connection formation when coupling contact is established between the coupling formation of the liquid supply interface and the counter-coupling formation of the at least one cell culture container.

9. The cell culture system according to claim 1 , wherein:
the signaling means and the valve body comprise a magnet, on one hand, and a ferromagnetic and/or magnetized component attracting its magnetic field, on the other hand, wherein the valve body is a valve body of the valve configuration, which can be shifted away from its valve seat and/or toward said seat for forming a sealing contact under the effect of the magnetic field between the signaling means and the valve body;
the valve configuration is pretensioned magnetically in the blocked position and is adjustable into the outlet position by the magnetic field starting from the signaling means;
a valve seat of the valve configuration has a permanent magnetic or ferromagnetic tension component, such that a magnetic tension force is in effect between the tension component and the valve body, which tensions the valve body at the valve seat for sealing contact;
the valve seat has an elastomer contact component, at which the valve body is directly positioned in the blocked position of the valve configuration, wherein the magnetic tensioning force in effect between the valve body and the tension component is an attracting force; and
in at least one connection formation, an annular axial end face of the elastomer contact comp

Assignees

• Hamilton Bonaduz Ag

Inventors

• JäGER THOMAS
• Schlenker Dirk
• Othman Nabih
• Kühne Oliver

Classifications

• C12M23/40 Primary

  Manifolds; Distribution pieces (fluid transfer means B01L3/563) · CPC title

• C12M41/00

  Means for regulation, monitoring, measurement or control, e.g. flow regulation (controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N) · CPC title

• C12M41/44 Primary

  of volume or liquid level · CPC title

• C12M33/07

  Dosage or metering devices therefore · CPC title

• C12M37/04

  Seals · CPC title