Method for removing biopolymer aggregates and viruses from a fluid

Patent metadata

Field	Value
Publication number	US-10987629-B2
Application number	US-201214125755-A
Country	US
Kind code	B2
Filing date	Apr 28, 2012
Priority date	Jun 24, 2011
Publication date	Apr 27, 2021
Grant date	Apr 27, 2021

Abstract

The present invention relates to a method for removing biopolymer aggregates and viruses from a fluid. In a first step, the biopolymer aggregates are selectively removed by filtration through a porous, polyamide-comprising shaped body having a native surface. In a second step, the biopolymer aggregate-free fluid is filtered through at least one suitable virus-retentive membrane.

First claim

The invention claimed is:

1. A method for removing biopolymer aggregates and parvoviruses from a fluid, comprising the steps of:
(a) filtering the fluid containing the biopolymer aggregates and parvoviruses through a porous, polyamide shaped body consisting of nylon-6, the porous, polyamide shaped body having internal and external surface regions and being configured so that all of the internal and external surfaces regions have the same chemical properties, the filtering of the fluid containing the biopolymer aggregates and parvoviruses through the porous, polyamide shaped body causing the biopolymer aggregates to be selectively depleted from the fluid by adsorption, whereas the parvoviruses permeate through the shaped body in a biopolymer aggregate-depleted fluid; and
(b) filtering the biopolymer aggregate-depleted fluid from step (a) through at least one parvovirus-retentive membrane having a molecular weight cut-off of from 100 to 1000 kD, wherein the content of parvoviruses in the fluid that has passed through the at least one parvovirus-retentive membrane is reduced by at least 99.9% with respect to the content of parvoviruses prior to carrying out step (a); wherein:
the biopolymer aggregates are selected from the group of the dimers, trimers or multimers of peptides, proteins, nucleic acids or mixtures thereof;
the fluid comprises a human blood plasma product, a protein solution obtained from a cell culture, a protein solution obtained from extraction of plant products, or a protein solution obtained from microorganisms;
the pH of the fluid during steps (a) and (b) is 9;
the porous, polyamide shaped body comprises a microporous membrane; and
the membrane used in step (b) comprises a material selected from the group consisting of polyethersulfone and polysulfone.

Assignees

• Thom Volkmar
• Hansmann Bjoern
• Sartorius Stedim Biotech Gmbh

Inventors

• Thom Volkmar
• Hansmann Bjoern

Classifications

• A61L2103/09

  Blood or products thereof · CPC title

• A61L2/022

  Filtration · CPC title

• A61L2103/05

  Living organisms or biological materials · CPC title

• B01D61/147

  Microfiltration · CPC title

• B01D61/145

  Ultrafiltration · CPC title